Human Serotonin N-acetyltransferase(AANAT) ELISA kit

說明書下載

中文名稱：

人芳烷基胺N-乙酰轉移酶(AANAT)酶聯免疫試劑盒
貨號：

CSB-EL001022HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人芳烷基胺N-乙酰轉移酶(AANAT)酶聯免疫試劑盒（CSB-EL001022HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的AANAT含量。AANAT（褪黑素合成酶）是褪黑素合成過程中的關鍵酶，受光周期調控，參與調節晝夜節律和多種生理過程。研究機制涉及褪黑素合成、晝夜節律調節及睡眠等生理功能的調控。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，科研人員可將其應用于神經生物學、時間生物學或內分泌學領域的機制研究，例如探究不同光照周期下 AANAT 的節律性表達、評估褪黑激素通路相關疾病的動物模型或細胞模型中酶活性變化，以及篩選影響生物鐘調控的小分子化合物。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

AA-NAT ELISA Kit; Aanat ELISA Kit; Aralkylamine N-acetyltransferase ELISA Kit; Arylalkylamine N acetyltransferase ELISA Kit; Serotonin acetylase ELISA Kit; Serotonin N-acetyltransferase ELISA Kit; SNAT ELISA Kit; SNAT_HUMAN ELISA Kit
縮寫：

AANAT
Uniprot No.：

Q16613
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AANAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	105
	Range %	103-109
1:2	Average %	96
	Range %	92-98
1:4	Average %	91
	Range %	86-96
1:8	Average %	93
	Range %	89-97

回收率：

The recovery of human AANAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-108
EDTA plasma (n=4)	92	87-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.141	2.302	2.222	2.121
1000	1.626	1.711	1.669	1.568
500	1.248	1.358	1.303	1.202
250	0.791	0.760	0.776	0.675
125	0.531	0.503	0.517	0.416
62.5	0.311	0.331	0.321	0.220
31.25	0.190	0.198	0.194	0.093
0	0.097	0.105	0.101

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
基因功能參考文獻：
1. Aggregate genetic variation in circadian rhythm and melatonin pathways were significantly associated with the risk of prostate cancer in data combining GAME-ON and PLCO, after Bonferroni correction (ppathway < 0.00625). The two most significant genes were NPAS2 (pgene = 0.0062) and AANAT (pgene = 0.00078); the latter being significant after Bonferroni correction. PMID: 28699174
2. Data show that arylalkymine N-acetyltransferase (AANAT) levels and melatonin synthesis change after transient receptor potential channel 4 (TRPV4 channel) stimulation in ciliary body epithelial cells. PMID: 28368307
3. these and related results indicate both a major involvement of the N-end rule pathway in the control of rodent AANATs and substantial differences in the regulation of rodent and human AANATs that stem from differences in their N-terminal sequences. PMID: 27339900
4. There is dysregulation of the AANAT/ASMT/melatonin --> melatonin receptor axis in cholangiocarcinoma, which inhibited melatonin secretion and subsequently enhanced CCA growth. PMID: 21778461
5. The expression of AANAT in epithelial cells of striated ducts in human submandibular glands. PMID: 21437622
6. The functional expression of human SNA protein was closely associated with the elevated synthesis of N-acetylserotonin and melatonin in transgenic rice plants. PMID: 20586889
7. Identified 17 sequence changes in AANAT gene of patients with major depression. Show evidence of the association of genetic variability in the AANAT gene with susceptibility to major depression. PMID: 20459461
8. There is a significant increase in AANAT allele positivity at the single nucleotide polymorphism (alanine 129--> threonine) at between patients with DSPS & controls. AA-NAT could be a susceptibility gene for DSPS. PMID: 12736803
9. Data suggest that the -263G/C single nucleotide polymorphism of arylalkylamine-N-acetyl-transferase (AA-NAT) may be an important determinant of the late/short sleep pattern. PMID: 15332344
10. Single nucleotide polymorphisms in the AANAT gene identified thus far cannot explain the observed interindividual differences for nocturnal melatonin profiles in the subjects investigated. PMID: 18569588
11. AANAT polymorphisms were not associated with adolescent idiopathic scoliosis. PMID: 18794762
12. Observational study of genotype prevalence and gene-disease association. (HuGE Navigator) PMID: 17503170
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亞細胞定位：

Cytoplasm.
蛋白家族：

Acetyltransferase family, AANAT subfamily
組織特異性：

Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.
數據庫鏈接：

HGNC: 19

OMIM: 600950

KEGG: hsa:15

STRING: 9606.ENSP00000250615

UniGene: Hs.431417

Human Serotonin N-acetyltransferase(AANAT) ELISA kit

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