Human Serine protease inhibitor Kazal-type 6(SPINK6) ELISA kit

說明書下載

中文名稱：

人絲氨酸蛋白酶抑制劑Kazal6型(SPINK6)酶聯免疫試劑盒
貨號：

CSB-EL022579HU
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

人絲氨酸蛋白酶抑制劑Kazal6型(SPINK6)酶聯免疫試劑盒（CSB-EL022579HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SPINK6含量。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，本試劑盒適用于探索其在皮膚疾病、組織修復及免疫調節中的分子機制，為皮膚病學、炎癥相關信號通路研究提供高效工具，滿足科研場景下對生物樣本中SPINK6蛋白的定量分析需求，助力于疾病機理、藥物靶點篩選等基礎研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Acrosin inhibitor ELISA Kit; BUSI2 ELISA Kit; EG433180 ELISA Kit; ISK6_HUMAN ELISA Kit; MGC21394 ELISA Kit; Protease inhibitor H ELISA Kit; Serine protease inhibitor Kazal-type 6 ELISA Kit; Spink5l1 ELISA Kit; SPINK6 ELISA Kit; UNQ844 ELISA Kit; UNQ844/PRO1782 ELISA Kit
縮寫：

SPINK6
Uniprot No.：

Q6UWN8
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SPINK6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	98
	Range %	94-102
1:4	Average %	102
	Range %	98-106
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of human SPINK6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-106
EDTA plasma (n=4)	104	88-110

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	1.712	1.812	1.762	1.626
20	1.352	1.366	1.359	1.223
10	0.966	0.954	0.960	0.824
5	0.587	0.598	0.593	0.457
2.5	0.463	0.448	0.456	0.320
1.25	0.323	0.345	0.334	0.198
0.625	0.217	0.221	0.219	0.083
0	0.135	0.136	0.136

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Serine protease inhibitor selective for kallikreins. Efficiently inhibits KLK4, KLK5, KLK6, KLK7, KLK12, KLK13 and KLK14. Doesn't inhibit KLK8.
基因功能參考文獻：
1. Study showed that the expression of SPINK6 is specifically suppressed in liver tumor tissues. The suppression could be even detected in the early stage tumors. As SPINK6 is a secretory protein, the extracellular protein level may be decreased because of tumor development. These results support that SPINK6 is an important tumor suppressor in liver. PMID: 27999203
2. our results identified a novel EGFR-activating mechanism in which SPINK6 has a critical role in promoting nasopharyngeal carcinoma metastasis PMID: 27671677
3. a significant fraction of SPINK6-sensitive proteases in healthy saliva and confirmed the ability of gingipains to inactivate SPINK6 under ex vivo conditions PMID: 27354280
4. KLKs. Thereby, beside the conserved binding mode, we identified an alternate binding mode which has so far not been observed for SPINK inhibitors. PMID: 26828269
5. Our study indicates that Spink6 is a potent inhibitor of kallikrein related peptidases and involved in skin barrier function. PMID: 24352040
6. Cross-linked SPINK6 might protect specific substrates such as fibronectin from kallikrein-related peptidases cleavage and contribute to the regulation of proteases in the epidermis. PMID: 23303447
7. Serine protease of Kazal-type (SPINK6) expressed in normal human skin is a potent natural inhibitor of Kallikrein-related peptidases, KLK12 and KLK13. PMID: 21439340
8. Data suggest that SPINK6 plays a role in modulating the activity of kallikreins in human skin. PMID: 20667819
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亞細胞定位：

Secreted.
數據庫鏈接：

HGNC: 29486

OMIM: 615868

KEGG: hsa:404203

STRING: 9606.ENSP00000324870

UniGene: Hs.334274

Human Serine protease inhibitor Kazal-type 6(SPINK6) ELISA kit

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