Human Serglycin(SRGN) ELISA kit
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中文名稱：人絲甘蛋白聚糖(SRGN)酶聯(lián)免疫試劑盒
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貨號：CSB-EL022664HU
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規(guī)格：96T/48T
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價格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人絲甘蛋白聚糖(SRGN)酶聯(lián)免疫試劑盒（CSB-EL022664HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SRGN含量。SRGN即聚硫蛋白聚糖，在多種生理和病理過程中發(fā)揮作用。它參與細(xì)胞黏附、遷移等過程，還與炎癥反應(yīng)相關(guān)。研究發(fā)現(xiàn)其在腫瘤微環(huán)境中可調(diào)節(jié)免疫細(xì)胞功能，影響腫瘤進(jìn)展，目前是腫瘤及免疫相關(guān)研究中值得關(guān)注的靶點(diǎn)。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于探索SRGN在免疫細(xì)胞功能研究、腫瘤微環(huán)境分析、炎癥性疾病模型構(gòu)建等科研場景中的表達(dá)特征與調(diào)控機(jī)制，為深入解析SRGN在疾病發(fā)生發(fā)展中的分子機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Chondroitin sulfate proteoglycan core protein ELISA Kit; Cytolytic granule proteoglycan core protein ELISA Kit; FLJ12930 ELISA Kit; gp600 ELISA Kit; Hematopoetic proteoglycan core protein ELISA Kit; Mastocytoma proteoglycan core protein ELISA Kit; MGC9289 ELISA Kit; OTTHUMP00000019716 ELISA Kit; P.PG ELISA Kit; PG19 core protein ELISA Kit; Pgsg ELISA Kit; Platelet proteoglycan core protein ELISA Kit; platelet proteoglycan protein core ELISA Kit; PLATELET PROTEOGLYCAN PROTEIN CORE; PPG ELISA Kit; PPG ELISA Kit; PRG ELISA Kit; PRG1 ELISA Kit; PROTEOGLYCAN 1 ELISA Kit; proteoglycan 1; secretory granule ELISA Kit; Proteoglycan 10K core protein ELISA Kit; Proteoglycan peptide core protein ELISA Kit; PROTEOGLYCAN PROTEIN CORE FOR MAST CELL SECRETORY GRANULE ELISA Kit; secretory granule proteoglycan 1 ELISA Kit; Secretory granule proteoglycan core peptide ELISA Kit; Secretory granule proteoglycan core protein ELISA Kit; Serglycin ELISA Kit; serglycin proteoglycan ELISA Kit; Sgc ELISA Kit; Srgn ELISA Kit; SRGN_HUMAN ELISA Kit
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縮寫：SRGN
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：0.312 ng/mL-20 ng/mL
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靈敏度：0.078 ng/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Cell Biology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human SRGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 107 Range % 103-112 1:2 Average % 97 Range % 93-102 1:4 Average % 94 Range % 88-98 1:8 Average % 96 Range % 92-99 -
回收率：
The recovery of human SRGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 88-97 EDTA plasma (n=4) 87 82-91 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 20 2.612 2.535 2.574 2.414 10 2.265 2.171 2.218 2.058 5 1.667 1.632 1.650 1.490 2.5 1.078 1.064 1.071 0.911 1.25 0.675 0.669 0.672 0.512 0.625 0.443 0.438 0.441 0.281 0.312 0.328 0.323 0.326 0.166 0 0.161 0.159 0.160 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- SRGN-mediated reactivation of the YAP/CRISPLD2 axis promotes aggressiveness of hepatocellular carcinoma S Zhang, H Hu, X Li, Q Chen, Y Zheng, H Peng, Z Mo,/,/
- Serological proteomic profiling uncovered CDK5RAP2 as a novel marker in benign prostatic hyperplasia H Xie, J Fan, J Wang, T Liu, L Chen, Y Pan, Y Li,Clinical Biochemistry,2024
- SRGN-TGF[beta]2 regulatory loop confers invasion and metastasis in triple-negative breast cancer Zhang Z.et al,Oncogenesis,2017
產(chǎn)品評價
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靶點(diǎn)詳情
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功能：Plays a role in formation of mast cell secretory granules and mediates storage of various compounds in secretory vesicles. Required for storage of some proteases in both connective tissue and mucosal mast cells and for storage of granzyme B in T-lymphocytes. Plays a role in localizing neutrophil elastase in azurophil granules of neutrophils. Mediates processing of MMP2. Plays a role in cytotoxic cell granule-mediated apoptosis by forming a complex with granzyme B which is delivered to cells by perforin to induce apoptosis. Regulates the secretion of TNF-alpha and may also regulate protease secretion. Inhibits bone mineralization.
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基因功能參考文獻(xiàn)：
- SRGN overexpression promoted colorectal cancer cell migration and invasion and SRGN was physically binding to a hypoxia response element in its promoter region. PMID: 30121667
- SRGN secreted by tumor cells and stromal components in the TME promotes malignant phenotypes through interacting with tumor cell receptor CD44 PMID: 27819672
- Extracellular spce serglycin upregulated CD44 receptor expression to maintain nasopharyngeal carcinoma stemness by interacting with CD44 and activating the MAPK/beta-catenin pathway. PMID: 27809309
- SRGN expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
- Patients with NPC with tumors showing strong tumor intensity and low infiltrated percentage of tumor-infiltrated lymphocytes with serglycin may be at high risk for distant metastases. PMID: 24995621
- The elevated levels of serglycin in aggressive cancer and stromal cells may suggest a key role for serglycin in disease progression PMID: 26581653
- These results suggest functions for serglycin in endothelial cells trough interactions with partner molecules, in biological processes with relevance for diabetic complications, cardiovascular disease and cancer development. PMID: 26694746
- These results suggest that different signaling pathways are involved in regulating secretion of serglycin and partner molecules in activated endothelial cells. PMID: 24513305
- The reduced expression of SRGN was accompanied by elevated levels of E-cadherin. PMID: 23996242
- These studies provide direct evidence for a critical role for serglycin in MM pathogenesis and show that targeting serglycin may provide a novel therapeutic approach for MM. PMID: 24403068
- These results suggest that human epicardial adipose tissue (EAT) has strong inflammatory properties in patients with coronary artery disease and provide novel evidence that serglycin is an adipocytokine highly expressed in EAT. PMID: 23376071
- Cell-surface SRGN promotes the adhesion of myeloma cells to collagen type I. PMID: 23387827
- This review covers recent studies on the involvement of serglycin in various pathological conditions, as well as its roles in immunity, hemostasis, cell growth, apoptosis, and reproduction. PMID: 22807344
- Human granulocyte precursors transfected with siRNA against serglycin displayed reduced capability to retain fully processed HNP-1. PMID: 21849484
- Serglycin regulates NPC metastasis via autocrine and paracrine routes, and that it serves as an independent prognostic indicator of metastasis-free survival and disease-free survival in NPC patients. PMID: 21289131
- Serglycin secreted by human multiple myeloma cell lines inhibits both the classical and lectin pathways of complement, without influencing alternative pathway activity. PMID: 21268013
- Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1. PMID: 21075844
- proapoptotic granzyme is exocytosed predominantly as a macromolecular complex with serglycin PMID: 12388539
- cell- and differentiation-specific alterations in chromatin structure may control serglycin gene expression PMID: 14506241
- serglycin-bound granzyme B in high-molecular-weight degranulate material from cytotoxic T lymphocytes predominantly followed a dynamin-dependent pathway to kill target cells PMID: 14739229
- mechanism of delivery is proposed entailing electrostatic transfer of granzyme B from serglycin to cell surface proteins PMID: 15788411
- Serglycin release is a constitutive process, which may be of fundamental biological importance in the study of multiple myeloma. PMID: 16870619
- Serglycin is of importance for secretory processes in human monocytes. PMID: 17909965
- Mutations in SRGN is not associated with familial hemophagocytic lymphohistiocytosis PMID: 18000860
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亞細(xì)胞定位：Cytoplasmic granule. Cytolytic granule. Secreted, extracellular space. Golgi apparatus.
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蛋白家族：Serglycin family
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