Human Secreted phosphoprotein 2 (SPP2/SPP24) ELISA kit
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中文名稱：人分泌磷蛋白2(SPP2/SPP24)酶聯(lián)免疫試劑盒
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貨號：CSB-EL022604HU
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規(guī)格：96T/48T
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價格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人分泌磷蛋白2(SPP2/SPP24)酶聯(lián)免疫試劑盒（CSB-EL022604HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SPP2含量。試劑盒檢測范圍為15.6 ng/ml-1000 ng/ml，適用于體外研究SPP2/SPP24在骨骼生物學、鈣磷代謝調(diào)節(jié)、組織礦化或相關(guān)疾病模型中的表達水平變化，為探索該蛋白的分子功能及病理關(guān)聯(lián)提供靈敏穩(wěn)定的科研工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：SPP2 ELISA Kit; SPP24 ELISA Kit; Secreted phosphoprotein 24 ELISA Kit; Spp-24 ELISA Kit; Secreted phosphoprotein 2 ELISA Kit
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縮寫：SPP2
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：15.6 ng/ml-1000 ng/ml
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靈敏度：3.9 ng/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Others
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human SPP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:5 Average % 98 Range % 96-102 1:10 Average % 107 Range % 103-114 1:20 Average % 94 Range % 90-98 1:40 Average % 88 Range % 86-95 -
回收率：
The recovery of human SPP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 105 99-108 EDTA plasma (n=4) 94 90-98 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 1000 2.188 2.233 2.211 2.028 500 1.732 1.824 1.778 1.595 250 1.211 1.261 1.236 1.053 125 0.698 0.757 0.728 0.545 62.5 0.523 0.522 0.523 0.340 31.2 0.345 0.358 0.352 0.169 15.6 0.252 0.260 0.256 0.073 0 0.176 0.189 0.183 . -
數(shù)據(jù)處理：
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貨期：3-5 working days
產(chǎn)品評價
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靶點詳情
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功能：Could coordinate an aspect of bone turnover.
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基因功能參考文獻：
- Spp24 can inhibit the growth of prostate cancer and its bone metastasis induced by BMP2; spp24 may have great potential to be a therapeutic agent in clinical situations PMID: 27793899
- the two mutations of SPP2 have dominant negative effects and cellular accumulation of Spp-24 might be particularly toxic to photoreceptors and/or retinal pigment epithelium. SPP2 has a new role in retinal degeneration. PMID: 26459573
- Report describes a C-terminal fragment within SPP24 that is distinct from the cystatin domain and which independently binds to BMP-2 and TGF-beta. This fragment inhibited BMP-2 activity in an ectopic bone forming assay. PMID: 25418420
- Spp24, or proteolytic products of Spp24, bind cytokines of the TGF-beta superfamily and also activate intracellular signaling pathways [review] PMID: 25339413
- Spp24 truncation products have effects on osteoblastic differentiation mediated by kinase pathways that are independent of exogenous BMP/TGF-beta cytokines. PMID: 25501958
- Results describe the localization of the human secreted phosphoprotein 24 gene, its structure and the start of transcription in liver. PMID: 15062857
- spp24 contains at least 2 single nucleotide polymorphisms. Given its mechanism of action and sequence variability, SPP24 may be an interesting candidate for future studies of the genetic regulation of bone mass. PMID: 19375587
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亞細胞定位：Secreted.
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蛋白家族：SPP2 family
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組織特異性：Detected in liver and plasma.
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數(shù)據(jù)庫鏈接：
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