Human Secreted phosphoprotein 2 (SPP2/SPP24) ELISA kit

說明書下載

中文名稱：

人分泌磷蛋白2(SPP2/SPP24)酶聯免疫試劑盒
貨號：

CSB-EL022604HU
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

人分泌磷蛋白2(SPP2/SPP24)酶聯免疫試劑盒（CSB-EL022604HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SPP2含量。試劑盒檢測范圍為15.6 ng/ml-1000 ng/ml，適用于體外研究SPP2/SPP24在骨骼生物學、鈣磷代謝調節、組織礦化或相關疾病模型中的表達水平變化，為探索該蛋白的分子功能及病理關聯提供靈敏穩定的科研工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

SPP2 ELISA Kit; SPP24 ELISA Kit; Secreted phosphoprotein 24 ELISA Kit; Spp-24 ELISA Kit; Secreted phosphoprotein 2 ELISA Kit
縮寫：

SPP2
Uniprot No.：

Q13103
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 ng/ml-1000 ng/ml
靈敏度：

3.9 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SPP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	98
	Range %	96-102
1:10	Average %	107
	Range %	103-114
1:20	Average %	94
	Range %	90-98
1:40	Average %	88
	Range %	86-95

回收率：

The recovery of human SPP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	99-108
EDTA plasma (n=4)	94	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.188	2.233	2.211	2.028
500	1.732	1.824	1.778	1.595
250	1.211	1.261	1.236	1.053
125	0.698	0.757	0.728	0.545
62.5	0.523	0.522	0.523	0.340
31.2	0.345	0.358	0.352	0.169
15.6	0.252	0.260	0.256	0.073
0	0.176	0.189	0.183
		.

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

產品評價

靶點詳情

功能：

Could coordinate an aspect of bone turnover.
基因功能參考文獻：
1. Spp24 can inhibit the growth of prostate cancer and its bone metastasis induced by BMP2; spp24 may have great potential to be a therapeutic agent in clinical situations PMID: 27793899
2. the two mutations of SPP2 have dominant negative effects and cellular accumulation of Spp-24 might be particularly toxic to photoreceptors and/or retinal pigment epithelium. SPP2 has a new role in retinal degeneration. PMID: 26459573
3. Report describes a C-terminal fragment within SPP24 that is distinct from the cystatin domain and which independently binds to BMP-2 and TGF-beta. This fragment inhibited BMP-2 activity in an ectopic bone forming assay. PMID: 25418420
4. Spp24, or proteolytic products of Spp24, bind cytokines of the TGF-beta superfamily and also activate intracellular signaling pathways [review] PMID: 25339413
5. Spp24 truncation products have effects on osteoblastic differentiation mediated by kinase pathways that are independent of exogenous BMP/TGF-beta cytokines. PMID: 25501958
6. Results describe the localization of the human secreted phosphoprotein 24 gene, its structure and the start of transcription in liver. PMID: 15062857
7. spp24 contains at least 2 single nucleotide polymorphisms. Given its mechanism of action and sequence variability, SPP24 may be an interesting candidate for future studies of the genetic regulation of bone mass. PMID: 19375587
顯示更多

收起更多
亞細胞定位：

Secreted.
蛋白家族：

SPP2 family
組織特異性：

Detected in liver and plasma.
數據庫鏈接：

HGNC: 11256

OMIM: 602637

KEGG: hsa:6694

STRING: 9606.ENSP00000168148

UniGene: Hs.444488

Human Secreted phosphoprotein 2 (SPP2/SPP24) ELISA kit

產品詳情

產品評價

相關產品

靶點詳情

Most popular with customers

產品中心

信號通路

技術信息

聯系信息