Human Regenerating islet-derived protein 3-gamma(REG3G) ELISA kit

Human Regenerating islet-derived protein 3-gamma(REG3G) ELISA kit

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中文名稱：

人再生胰島衍生蛋白質3-γ(REG3G)酶聯免疫試劑盒
貨號：

CSB-EL019549HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人再生胰島衍生蛋白質3-γ(REG3G)酶聯免疫試劑盒（CSB-EL019549HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的REG3G含量。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，該產品為科研領域提供可靠工具，支持感染性疾病模型、胰腺炎機制研究、糖尿病相關代謝調控分析以及腸道炎癥與微生物互作等基礎研究，尤其適用于評估組織損傷修復過程中REG3G的動態表達特征。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

LPPM429 ELISA Kit; Pancreatitis-associated protein 1B ELISA Kit; Pancreatitis-associated protein IB ELISA Kit; PAP IB ELISA Kit; PAP-1B ELISA Kit; PAP1B ELISA Kit; PAPIB ELISA Kit; REG III ELISA Kit; Reg III-gamma ELISA Kit; REG-3-gamma ELISA Kit; REG3G ELISA Kit; REG3G_HUMAN ELISA Kit; Regenerating islet derived 3 gamma ELISA Kit; Regenerating islet-derived protein 3-gamma ELISA Kit; Regenerating islet-derived protein III-gamma ELISA Kit; UNQ429 ELISA Kit
縮寫：

REG3G
Uniprot No.：

Q6UW15
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human REG3G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-97
1:2	Average %	99
	Range %	95-107
1:4	Average %	104
	Range %	100-110
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of human REG3G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	90	85-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.519	2.352	2.436	2.296
100	1.923	1.856	1.890	1.750
50	1.301	1.254	1.278	1.138
25	0.719	0.721	0.720	0.580
12.5	0.415	0.387	0.401	0.261
6.25	0.324	0.307	0.316	0.176
3.12	0.215	0.229	0.222	0.082
0	0.142	0.137	0.140

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.
基因功能參考文獻：
1. This gene encodes a secreted peptide that displays antimicrobial activity against Gram-positive bacteria. PMID: 16931762
2. a model of CagA-directed REG3gamma expression in gastric epithelial cells via activation of the IL-11/gp130/STAT3 pathway PMID: 22312430
3. REG III was identified and expressed predominantly in pancreas and testis, but not in small intestine PMID: 15556304
4. PAP IB represents a good example of duplication and divergence, probably with the acquisition of new functions, thus participating in the evolution of the protein repertoire. PMID: 15777617
亞細胞定位：

Secreted. Cytoplasm.
組織特異性：

Predominantly expressed in pancreas, where it may be restricted to exocrine pancreas. Moderate expression levels in testis and weak in heart, kidney and placenta.
數據庫鏈接：

HGNC: 29595

OMIM: 609933

KEGG: hsa:130120

STRING: 9606.ENSP00000272324

UniGene: Hs.447084

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.