Human Protein disulfide-isomerase A4(PDIA4) ELISA kit

Human Protein disulfide-isomerase A4(PDIA4) ELISA kit

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中文名稱：

人蛋白質二硫鍵異構酶A4(PDIA4)酶聯免疫試劑盒
貨號：

CSB-EL017722HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人蛋白質二硫鍵異構酶A4(PDIA4)酶聯免疫試劑盒（CSB-EL017722HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的PDIA4含量。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，本產品適用于基礎科研中PDIA4相關分子機制探索，包括但不限于內質網應激模型構建、氧化還原信號通路研究以及疾病相關生物標志物篩選等實驗場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Calcium binding protein intestinal related ELISA Kit; Endoplasmic reticulum resident protein 70 ELISA Kit; Endoplasmic reticulum resident protein 72 ELISA Kit; EPR70 ELISA Kit; EPR72 ELISA Kit; ER protein 70 ELISA Kit; ER protein 72 ELISA Kit; ERP 70 ELISA Kit; ERp 72 ELISA Kit; ERp-72 ELISA Kit; ERp70 ELISA Kit; ERp72 ELISA Kit; PDIA 4 ELISA Kit; Pdia4 ELISA Kit; PDIA4_HUMAN ELISA Kit; PDIR ELISA Kit; Protein disulfide isomerase A4 ELISA Kit; Protein disulfide isomerase associated 4 ELISA Kit; Protein disulfide isomerase family A member 4 ELISA Kit; protein disulfide isomerase related protein (calcium-binding protein; intestinal-related) ELISA Kit; Protein disulfide isomerase related protein ELISA Kit; Protein disulfide-isomerase A4 ELISA Kit; Protein ERp 72 ELISA Kit; Protein ERp72 ELISA Kit
縮寫：

PDIA4
Uniprot No.：

P13667
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

23.5 pg/ml-1500 pg/ml
靈敏度：

5.8 pg/ml
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PDIA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-97
1:2	Average %	100
	Range %	96-104
1:4	Average %	90
	Range %	88-93
1:8	Average %	91
	Range %	86-94

回收率：

The recovery of human PDIA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-94
EDTA plasma (n=4)	104	99-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	1.960	1.977	1.969	1.817
750	1.284	1.300	1.292	1.140
375	0.725	0.713	0.719	0.567
187.5	0.412	0.406	0.409	0.257
94	0.287	0.277	0.282	0.130
47	0.220	0.215	0.218	0.066
23.5	0.184	0.178	0.181	0.029
0	0.150	0.154	0.152

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

基因功能參考文獻：
1. up-regulated by IL11 in first trimester placenta PMID: 28487027
2. PDIA4 as a negative regulator of cancer cell apoptosis. PMID: 28534513
3. Participants with metabolic syndrome (MetS) had significantly higher serum PDIA4 levels than those without MetS (P<0.001). After adjustments, the individuals with the highest PDIA4 tertile were associated with a higher risk of MetS than those with the lowest tertile (OR = 4.83, 95% CI: 2.71-8.60). PMID: 28650993
4. CDDP-resistant non-small lung cancer cells undergo profound remodeling of their endoplasmic reticulum (ER) proteome (>80 proteins identified by proteomics) and exhibit a dramatic overexpression of two protein disulfide isomerases, PDIA4 and PDIA6. PMID: 24464223
5. novel esophageal squamous cell carcinoma marker PDIA4 was identified by mass spectrometry and immunohistochemical analysis PMID: 21743296
6. Nox1 associates with ERp72, which involves its N-terminus encompassing a Ca(2+)-binding site and the first TRX-like motif. PMID: 18620548
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亞細胞定位：

Endoplasmic reticulum lumen. Melanosome.
蛋白家族：

Protein disulfide isomerase family
數據庫鏈接：

HGNC: 30167

KEGG: hsa:9601

STRING: 9606.ENSP00000286091

UniGene: Hs.93659

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.