Human Protein FAM13A(FAM13A) ELISA kit

說明書下載

中文名稱：

人FAM13A蛋白(FAM13A)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL008075HU
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人FAM13A蛋白(FAM13A)酶聯(lián)免疫試劑盒（CSB-EL008075HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的FAM13A含量。試劑盒檢測(cè)范圍為23.44 pg/mL-1500 pg/mL，適用于體外研究FAM13A在疾病模型中的表達(dá)變化、分子機(jī)制探索及生物標(biāo)志物篩選，如細(xì)胞實(shí)驗(yàn)后的培養(yǎng)上清液檢測(cè)、動(dòng)物模型組織樣本分析或臨床前研究中的體液樣本定量，為呼吸道疾病、代謝綜合征等領(lǐng)域的科研工作提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

FAM13A ELISA Kit; FAM13A1 ELISA Kit; KIAA0914Protein FAM13A ELISA Kit
縮寫：

FAM13A
Uniprot No.：

O94988
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

23.44 pg/mL-1500 pg/mL
靈敏度：

5.86 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FAM13A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	101
	Range %	88-105
1:2	Average %	103
	Range %	97-108
1:4	Average %	94
	Range %	88-99
1:8	Average %	105
	Range %	94-108

回收率：

The recovery of human FAM13A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-104
EDTA plasma (n=4)	102	94-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.467	2.402	2.435	2.299
750	1.699	1.619	1.659	1.523
375	1.045	1.073	1.059	0.923
187.5	0.585	0.546	0.566	0.430
93.75	0.378	0.355	0.367	0.231
46.88	0.238	0.231	0.235	0.099
23.44	0.213	0.220	0.217	0.081
0	0.138	0.134	0.136

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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■ 常見問題解答

Per customers request, please provide the following information regarding Human Protein FAM13A, FAM13A ELISA Kit (CSB-EL008075HU)
1. What is the Uniprot/Accession number that this kit is designed for? Is the kit designed for XYZ species or is it designed for a different species such as Human and cross reacts with XYZ species?
2. Is the capture antibody a monoclonal or polyclonal?
3. What is the host of the capture antibody?
4. What’s the immunogen or immunogen range of the capture antibody?
5. What’s is the species of the immunogen of the capture antibody, human or something else?
6. Is the detection antibody a monoclonal or polyclonal?
7. What is the host of the detection antibody?
8. What is the immunogen or immunogen range of the detection antibody?
9. What is the species of the immunogen of the detection antibody? Human or something else?
10. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
11. If recombinant protein, is it full length or partial?
12. What is the species of the standard– human or something else?
13. What sample type was tested with this kit (serum? plasma? Something else?) and what were the reference values.

Thanks for your inquiry!
For Protein FAM13A, the protein sequence and the structure are different for different species.
CSB-EL008075HU
This kit is designed according to: https://www.uniprot.org/uniprot/O94988. It is specific to human.
Capture antibody is mouse monoclonal antibody and immunogen is NS0-derived recombinant Human Protein FAM13A protein.
Detection antibody is goat polyclonal antibody and immunogen is NS0-derived recombinant Human Protein FAM13A protein.
Standard is NS0-derived recombinant Human Protein FAM13A protein.
We tested undiluted serum and plasma samples before and the test value is 150--400pg/ml.
Pls let me know if you have any further questions. Thank you.

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Human Protein FAM13A(FAM13A) ELISA kit

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