Human Proadrenomedullin (Pro-ADM) ELISA Kit

產品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人腎上腺髓質素前體(Pro-ADM)酶聯免疫試劑盒（CSB-E14356h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、尿液、組織勻漿樣本中的Pro-ADM含量。人腎上腺髓質素前體（Pro-ADM）是一種由多種組織產生的肽，具有穩定微循環和防止內皮通透性的作用。其在膿毒癥、下呼吸道感染等疾病中顯示出作為治療監測和風險評估的潛力，其水平與疾病嚴重程度和后續不良事件的可能性相關。試劑盒檢測范圍為0.312 pmol/L-20 pmol/L，該產品支持多種生物樣本類型，能夠滿足基礎醫學研究中疾病機制探索、藥物作用評估或生物標志物篩選等需求，例如通過檢測循環系統中Pro - ADM的動態變化，為心血管疾病或全身性炎癥相關研究提供數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

Pro-ADM
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, urine, tissue homogenates
檢測范圍：

0.312 pmol/L-20 pmol/L
靈敏度：

0.078 pmol/L
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ADM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	85
	Range %	80-92
1:400	Average %	95
	Range %	90-100
1:800	Average %	99
	Range %	92-110
1:1600	Average %	92
	Range %	82-105

回收率：

The recovery of human Pro-ADM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-100
EDTA plasma (n=4)	101	90-110

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.