Human Peptidyl-prolyl cis-trans isomerase C(PPIC) ELISA kit

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中文名稱：

人肽脯氨酰順反異構(gòu)酶C(PPIC)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL018473HU
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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人肽脯氨酰順反異構(gòu)酶C(PPIC)酶聯(lián)免疫試劑盒（CSB-EL018473HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PPIC含量。人肽脯氨酰順反異構(gòu)酶C（PPIC）是一種在蛋白質(zhì)折疊和成熟中起關(guān)鍵作用的酶，通過催化脯氨酸酰亞胺肽鍵的順反異構(gòu)化，確保蛋白質(zhì)正確折疊和成熟，對(duì)細(xì)胞蛋白質(zhì)穩(wěn)態(tài)至關(guān)重要。試劑盒檢測(cè)范圍為23.5 pg/mL-1500 pg/mL，適用于基礎(chǔ)科研中PPIC相關(guān)疾病機(jī)制探索、細(xì)胞模型或動(dòng)物模型的蛋白表達(dá)動(dòng)態(tài)分析，以及藥物干預(yù)實(shí)驗(yàn)中的生物標(biāo)志物定量評(píng)估?？蔀榉肿由飳W(xué)、病理學(xué)及藥理學(xué)研究提供可靠的數(shù)據(jù)支持，滿足科研工作者對(duì)PPIC蛋白定量分析的多樣化需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Cyclophilin C ELISA Kit; CYPC ELISA Kit; MGC3673 ELISA Kit; Parvulin ELISA Kit; Peptidyl prolyl cis trans isomerase C ELISA Kit; Peptidyl-prolyl cis-trans isomerase C ELISA Kit; Peptidylprolyl isomerase C (cyclophilin C) ELISA Kit; peptidylprolyl isomerase C ELISA Kit; PPIase C ELISA Kit; ppiC ELISA Kit; PPIC_HUMAN ELISA Kit; Rotamase C ELISA Kit
縮寫：

PPIC
Uniprot No.：

P45877
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

23.5 pg/mL-1500 pg/mL
靈敏度：

5.8 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PPIC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-104
1:2	Average %	91
	Range %	82-98
1:4	Average %	103
	Range %	98-108
1:8	Average %	87
	Range %	82-98

回收率：

The recovery of human PPIC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-98
EDTA plasma (n=4)	111	105-115

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.651	2.656	2.654	2.497
750	2.322	2.251	2.287	2.130
375	1.645	1.655	1.650	1.493
187.5	1.002	0.974	0.988	0.831
94	0.587	0.544	0.566	0.409
47	0.389	0.401	0.395	0.238
23.5	0.262	0.245	0.254	0.097
0	0.156	0.157	0.157

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

引用文獻(xiàn)

Increase of serum Cyclophilin C levels in the follow-up of coronary artery disease: A biomarker and possible clinical predictor J Bayón,Archivos de cardiologia de Mexico,2023
Cyclophilins A, B, and C Role in Human T Lymphocytes Upon Inflammatory Conditions S Gegunde,Frontiers in Immunology,2021
Cyclophilins in Ischemic Heart Disease: Differences Between Acute and Chronic Coronary Artery Disease Patients J Bayon,Cardiol Res Pract,2020
High serum Cyclophilin C levels as a risk factor marker for Coronary Artery Disease MA Alfonso Ranca帽o,Sci Rep,2019
High Serum Cyclophilin C levels as a risk factor marker for Coronary Artery Disease Alfonso A, et al,Scientific Reports,2019

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功能：

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.
基因功能參考文獻(xiàn)：
1. Independent validation with glioma patients tissue (n = 70) and normal brain tissue (n = 19) found PPIC, EMP3 and CHI3L1 were up-regulated in glioma tissue. Survival value validation showed that the three genes correlated with patient survival by Kaplan-Meir analysis, including grades, age and therapy. PMID: 27801851
2. Cyclophilin C participates in cytomegalovirus US2 protein-mediated degradation of major histocompatibility complex class I molecules. PMID: 26691022
亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

Cyclophilin-type PPIase family
組織特異性：

Expressed in kidney, skeletal muscle, pancreas, heart, lung, liver and to a lower extent in brain.
數(shù)據(jù)庫鏈接：

HGNC: 9256

OMIM: 123842

KEGG: hsa:5480

STRING: 9606.ENSP00000303057

UniGene: Hs.110364

Human Peptidyl-prolyl cis-trans isomerase C(PPIC) ELISA kit

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