Human Pepsinogen II ELISA Kit

產品詳情

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文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人胃蛋白酶原II酶聯免疫試劑盒（CSB-E17539h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液樣本中的PG II含量。PG II即胃蛋白酶原II，是胃蛋白酶的前體。其背景在于與胃部健康密切相關，可反映胃黏膜狀態。研究機制方面，通過檢測血清中PG II水平，能輔助診斷胃部疾病，如胃潰瘍、胃癌等，為胃腸病的早期篩查提供重要依據。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，適用于基礎醫學研究領域，包括胃黏膜功能評估、胃酸分泌調控機制研究、胃部疾病動物模型構建等科研場景，同時可應用于體外培養細胞分泌功能檢測，為探索胃蛋白酶原II在消化系統疾病中的分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PG II
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates
檢測范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

1.56 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human pepsinogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	96-107
1:2	Average %	94
	Range %	90-98
1:4	Average %	95
	Range %	89-99
1:8	Average %	93
	Range %	87-98

回收率：

The recovery of human pepsinogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	86	81-92

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.