Human Pepsinogen I ELISA Kit

Human Pepsinogen I ELISA Kit

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中文名稱：

人胃蛋白酶原I酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E17538h
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人胃蛋白酶原I酶聯(lián)免疫試劑盒（CSB-E17538h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PG I含量。PG I（胃蛋白酶原I）是胃中的一種消化酶，其生物學(xué)功能包括消化食物中的蛋白質(zhì)。研究顯示，PG I在心血管系統(tǒng)中具有重要作用，其合成能力由內(nèi)向外依次減弱，以內(nèi)皮細(xì)胞合成能力最強(qiáng)。PG I的研究機(jī)制涉及其對(duì)心血管系統(tǒng)的影響，以及其在胃癌診斷中的應(yīng)用。試劑盒檢測(cè)范圍為6.25 ng/mL-400 ng/mL，支持科研人員高效開展胃功能研究、胃黏膜損傷機(jī)制探索或藥物干預(yù)效果評(píng)估等實(shí)驗(yàn)。其寬泛的線性范圍和多類型樣本兼容性，特別適用于縱向研究或不同實(shí)驗(yàn)條件間的數(shù)據(jù)比對(duì)，為胃相關(guān)疾病的分子機(jī)制解析及生物標(biāo)志物篩選提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PG I
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

1.56 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human pepsinogen I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	106
	Range %	100-115
1:4	Average %	97
	Range %	94-112
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of human pepsinogen I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	97	90-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
400	2.396	2.252	2.324	2.207
200	1.612	1.736	1.674	1.557
100	1.104	1.054	1.079	0.962
50	0.662	0.639	0.651	0.534
25	0.399	0.424	0.412	0.295
12.5	0.292	0.309	0.301	0.184
6.25	0.205	0.199	0.202	0.085
0	0.118	0.115	0.117

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Evaluation of serum pepsinogen concentration for the diagnosis of type 1 abomasal ulcer in water buffalo SA Hussain,Comparative Clinical Pathology,2023
Comparative Effectiveness of Probiotics Timing Regimen in Helicobacter pylori-Induced Peptic Ulcer Disease Patients Aseel Mohammed Ameen, et al,Journal Of Pharmacological Sciences,2019
Helicobacter pylori Stool Antigen Levels and Serological Biomarkers of Gastric Inflammation are Associated with Cardio-Metabolic Risk Factors in Type 2 Diabetic Patients. Bahadoran Z.et al,Endocrinol Metab (Seoul).,2015
Complementary and alternative medicinal effects of broccoli sprouts powder on Helicobacter pylori eradication rate in type 2 diabetic patients: A randomized Zahra Bahadoran et al,Journal of Functional Foods,2014
Down-regulation of Phosphoglucose Isomerase/Autocrine Motility Factor Enhances Gensenoside Rh2 Pharmacological Action on Leukemia KG1a Cells You ZM et al,Asian Pac J Cancer Prev,2014

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.