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Human Olfactomedin-like protein 2B(OLFML2B) ELISA kit

說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

人嗅覺素樣蛋白2B(OLFML2B)酶聯(lián)免疫試劑盒（CSB-EL016326HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的OLFML2B含量。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，科研人員可通過該產(chǎn)品研究OLFML2B在不同生理或病理?xiàng)l件下的表達(dá)調(diào)控，例如探究其在特定組織中的分布特征、疾病動物模型中的動態(tài)變化，或與相關(guān)信號通路的潛在關(guān)聯(lián)性，為神經(jīng)生物學(xué)、發(fā)育調(diào)控及疾病機(jī)制研究提供重要實(shí)驗(yàn)依據(jù)，助力新型生物標(biāo)志物的功能探索。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

OLFML2B ELISA Kit; Olfactomedin-like protein 2B ELISA Kit; Photomedin-2 ELISA Kit
縮寫：

OLFML2B
Uniprot No.：

Q68BL8
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human OLFML2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	87-98
1:2	Average %	101
	Range %	98-104
1:4	Average %	94
	Range %	87-98
1:8	Average %	92
	Range %	89-97

回收率：

The recovery of human OLFML2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-99
EDTA plasma (n=4)	92	90-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.