Human Nidogen 1 (NID1) ELISA Kit

說明書下載

中文名稱：

人巢蛋白1(NID1)酶聯免疫試劑盒
貨號：

CSB-E13289h
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

人巢蛋白1(NID1)酶聯免疫試劑盒（CSB-E13289h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NID1含量。試劑盒檢測范圍為0.39 ng/mL-25 ng/mL，靈敏度為0.09 ng/mL。適用于基礎科研中探索NID1在腫瘤微環境調控、纖維化疾病進程或再生醫學中的作用機制，也可用于藥物開發模型中評估靶向細胞外基質的干預效果。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Enactin ELISA Kit; Ent ELISA Kit; Entactin 1 ELISA Kit; Entactin ELISA Kit; NID 1 ELISA Kit; NID ELISA Kit; NID-1 ELISA Kit; Nid1 ELISA Kit; NID1_HUMAN ELISA Kit; Nidogen (enactin) ELISA Kit; Nidogen 1 ELISA Kit; Nidogen ELISA Kit; Nidogen-1 ELISA Kit
縮寫：

NID1
Uniprot No.：

P14543
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates
檢測范圍：

0.39 ng/mL-25 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	3.046	3.371	2.917	3.287	3.039	3.399
SD	0.038	0.035	0.04	0.055	0.049	0.057
CV(%)	4.455	3.885	4.802	6.187	5.751	6.298

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human NID 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

82-93

1:2

Average %

Range %

94-100

1:4

Average %

Range %

90-104

1:8

Average %

Range %

82-93

回收率：

The recovery of human NID 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	101	96-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.











ng/ml	OD1	OD2	Average	Corrected
25	2.186	2.140	2.163	2.016
12.5	1.642	1.633	1.638	1.491
6.25	1.251	1.220	1.236	1.089
3.12	0.873	0.830	0.852	0.705
1.56	0.579	0.541	0.560	0.413
0.78	0.332	0.342	0.337	0.190
0.39	0.260	0.265	0.263	0.116
0	0.148	0.145	0.147

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
基因功能參考文獻：
1. Results demonstrated that NID1 was a mesenchymal associated gene and its high expression was significantly correlated with shorter overall survival of ovarian cancer patients. The study presents evidence that NID1 promotes the migration, invasion and chemoresistance of ovarian cancer cells via EMT and its molecular mechanism involves the activation of ERK/MAPK signaling. PMID: 28416770
2. Nidogen 1 (NID1) was confirmed to promote lung metastasis of breast cancer and melanoma. PMID: 28827399
3. nidogen-1 degraded by CatS can be quantified in serum by the NIC assay. The current data strongly suggest that cathepsin-S degradation of nidogen-1 is strongly associated with NSCLC, which needs validation in larger clinical cohorts. PMID: 28282545
4. Differential protein expression of collagen IV, laminin alpha2, and nidogen-1 indicated basal lamina remodeling develops in ischemic failing versus nonfailing human hearts. PMID: 26756417
5. NID1 was confirmed as a target gene of MiR-192/215 in Hirschsprung's disease patients. PMID: 25857602
6. Increase of NID1 protein expression is associated with endometrial cancer invasion. PMID: 25924802
7. The cleavage of nidogen-1 and -2 is partially inhibited in human tumor samples. PMID: 23681936
8. results demonstrate that mutation in either NID1 or LAMC1 disrupts the interaction NID1-LAMC1 complex; findings implicate the extracellular matrix in the pathogenesis of Dandy-Walker spectrum disorders PMID: 23674478
9. Cleavage of nidogen-1 by cathepsin S impairs its binding to basement membrane partners PMID: 22952693
10. NID1 gene is a biologically plausible locus for nevogenesis and melanoma development PMID: 21478494
11. found to be ubiquitous component of basement membrane zones underneath developing epithelia of most of the major organ systems; may participate in cell biological functions during human development PMID: 12005023
12. nidogen-2 is equivalent to nidogen-1 in skin-organotypic coculture, and both can promote the development of a functional basement membrane zone PMID: 17008882
13. CpG islands of both NID1 and NID2 genes are aberrantly methylated in human cancer samples and cancer cell lines. PMID: 17328794
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亞細胞定位：

Secreted, extracellular space, extracellular matrix, basement membrane.
數據庫鏈接：

HGNC: 7821

OMIM: 131390

KEGG: hsa:4811

STRING: 9606.ENSP00000264187

UniGene: Hs.356624

Human Nidogen 1 (NID1) ELISA Kit

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