Human Neutrophil defensin 3(DEFA3) ELISA kit

Human Neutrophil defensin 3(DEFA3) ELISA kit

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中文名稱：

人中性粒細胞防御素3(DEFA3)酶聯免疫試劑盒
貨號：

CSB-EL006655HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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人中性粒細胞防御素3(DEFA3)酶聯免疫試劑盒（CSB-EL006655HU）檢測血清、血漿,組織勻漿樣本中的DEFA3含量。DEFA3是一種防御素，存在于人體中性粒細胞中，具有抗菌、抗病毒和免疫調節等功能。研究機制方面，其通過與病原體相互作用，破壞病原體的膜結構，影響其生存與繁殖，還能調節免疫細胞活性，在機體抗感染免疫中發揮重要作用。試劑盒檢測范圍為1.87 ng/mL-120 ng/mL，本產品適用于科研領域對DEFA3表達水平的定量研究，例如探索感染性疾病中宿主防御機制、炎癥相關疾病的分子調控路徑，或評估不同實驗條件下中性粒細胞功能狀態。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

DEFA3 ELISA Kit; DEF3Neutrophil defensin 3 ELISA Kit; Defensin ELISA Kit; alpha 3 ELISA Kit; HNP-3 ELISA Kit; HP-3 ELISA Kit; HP3) [Cleaved into: HP 3-56; Neutrophil defensin 2 ELISA Kit; HNP-2 ELISA Kit; HP-2 ELISA Kit; HP2)] ELISA Kit
縮寫：

DEFA3
Uniprot No.：

P59666
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma,tissue homogenates
檢測范圍：

1.87 ng/mL-120 ng/mL
靈敏度：

0.47 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DEFA3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
Range %	88-101
1:2	Average %	92
Range %	84-96
1:4	Average %	94
Range %	85-99
1:8	Average %	98
Range %	90-102

回收率：

The recovery of human DEFA3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	89-104
EDTA plasma (n=4)	98	90-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
120	1.875	1.897	1.886	1.762
60	1.412	1.476	1.444	1.320
30	0.907	0.928	0.918	0.794
15	0.552	0.545	0.549	0.425
7.5	0.359	0.344	0.352	0.228
3.75	0.245	0.241	0.243	0.119
1.87	0.185	0.179	0.182	0.058
0	0.125	0.123	0.124

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Potential Association of Gut Microbial Metabolism and Circulating mRNA Based on Multiomics Sequencing Analysis in Fetal Growth Restriction H Tang,Mediators of inflammation,2024

功能：

Defensin 2 and defensin 3 have antibiotic, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.
基因功能參考文獻：
1. High HNP3 expression is associated with IgA Nephropathy. PMID: 27563166
2. Elevated DEFA3 levels in diabetes are independent of DEFA3 copy numbers. PMID: 25083086
3. DEFA3 encodes an antibacterial peptide that is bacteriocidal against S. aureus, E. coli, and P. aeruginosa. PMID: 2997278
4. alpha-Defensin (DEFA3)was the third most differentially overexpressed gene and may be related to the onset of Bell's palsy and Ramsay Hunt Syndrome. PMID: 22737966
5. The total amount of gingival crevicular fluid human neutrophil peptide 3 were not different among periodontitis, gingivitis, and health control groups; there was no correlation with clinical periodontal parameters. PMID: 20151808
6. BPI and HNP 1-3 are accumulated in the synovial cavity of patients with rheumatoid arthritis PMID: 12913926
7. Aerobic bacteria were 100% susceptible to HBD-2 and HBD-3, whereas only 21.4 and 50% of the anaerobes were susceptible to HBD-2 and HBD-3. PMID: 15004048
8. HNP-3 is a potentially important regulator of neovascularizaiton, suggesting a new link between inflammation and angiogenesis. PMID: 15208269
9. We have tested the DEFA3 absence in 697 samples from different human populations. The proportion of subjects lacking DEFA3 varies from 10% to 37%, depending on the population tested, suggesting differences in innate immune function between populations. PMID: 17214878
10. Clostridium difficile toxin B interacts with high affinity with HNP-3 which may provide a defense mechanism against clostridial glucosylating cytotoxins. PMID: 18435932
11. Upregulation of HNP3 is associated with colorectal adenomas and carcinomas. PMID: 18957723
12. Salivary HNP-3concentrations increased following exercise PMID: 19263072
13. DEFA3 was upregulated in IPF patients with acute exacerbation PMID: 19363140
14. Studies indicate alpha-defensin, the products of neutrophils wase upregulated at the mRNA and protein levels in SLE patients. PMID: 19758174
15. Plasma levels of alpha-defensins 1-3 are an indicator of neutrophil activation in pregnant and post-partum women. PMID: 17845323
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亞細胞定位：

Secreted.
蛋白家族：

Alpha-defensin family
數據庫鏈接：

HGNC: 2762

OMIM: 604522

KEGG: hsa:1668

STRING: 9606.ENSP00000328359

UniGene: Hs.654448

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.