Human Neurturin(NRTN) ELISA kit

Human Neurturin(NRTN) ELISA kit

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中文名稱：

人神經秩蛋白(NRTN)酶聯免疫試劑盒
貨號：

CSB-EL016095HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人神經秩蛋白(NRTN)酶聯免疫試劑盒（CSB-EL016095HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NRTN含量。NRTN即神經調節素，屬于膠質細胞系源性神經營養因子家族。其可通過與受體相互作用，激活下游信號通路，在神經系統發育、維持神經細胞存活與功能方面發揮重要作用。目前圍繞其開展的研究，有望為神經系統疾病治療提供新方向。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，適用于體外研究神經退行性疾病模型中NRTN的表達動態、藥物干預對神經營養因子分泌的影響，或探索神經再生相關機制等科研用途本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Neurturin ELISA Kit; Neurturin precursor ELISA Kit; NRTN ELISA Kit; NRTN_HUMAN ELISA Kit; NTN ELISA Kit; prepro-neurturin ELISA Kit
縮寫：

NRTN
Uniprot No.：

Q99748
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

125 pg/mL-8000 pg/mL
靈敏度：

31.25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human NRTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-97
1:2	Average %	103
	Range %	97-108
1:4	Average %	97
	Range %	92-102
1:8	Average %	94
	Range %	89-99

回收率：

The recovery of human NRTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-97
EDTA plasma (n=4)	104	98-109

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.546	2.664	2.605	2.470
4000	2.196	2.075	2.136	2.001
2000	1.464	1.489	1.477	1.342
1000	0.836	0.822	0.829	0.694
500	0.428	0.433	0.431	0.296
250	0.283	0.294	0.289	0.154
125	0.196	0.190	0.193	0.058
0	0.136	0.134	0.135

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Effect of NTN and Lmx1α on the Notch Signaling Pathway during the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Dopaminergic Neuron-Like Cells Jinhua Zhang,Parkinson’s Disease,2021

功能：

Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells.
基因功能參考文獻：
1. biophysical results show that the relative concentration of GFRa2 on cell surfaces can affect the functional affinity of NRTN through avidity effects. PMID: 29414779
2. In the cochlea, NTN immunostaining was found in the supporting cells of organ of Corti, including Deiters' cells, Hensen cells as well as Claudius' cells. In the spiral ganglia, NTN was seen in both the cell bodies and the nerve fibers of neurons. PMID: 24139947
3. Neurturin contributes toward an aggressive cancer cell phenotype, neuropathic pain and neuronal plasticity in pancreatic cancer. PMID: 24067900
4. Human neurturin protected crayfish neurons and glia from photodynamic injury. PMID: 22847529
5. Cyclic AMP signalling through PKA but not Epac is essential for neurturin-induced biphasic ERK1/2 activation and neurite outgrowths through GFRalpha2 isoforms. PMID: 21723942
6. GDNF and NRTN are new neuromodulators that regulate the development of the neuromuscular synapse PMID: 11790765
7. The gene expression of protein was studied in the developing human tooth. PMID: 12397373
8. The results obtained suggest the involvement of NTN, PSP, and ART in processes subserving both the organization of this cortical region during development and the functional activity and maintenance of the mature human hippocampal neurons. PMID: 15829225
9. Addition of neurturin to activated peripheral blood mononuclear leukocytes reduces the amount of detectable tumor necrosis factor protein without altering its transcription. PMID: 16081799
10. GDNF and NTN differentially regulate the expression of distinct miRNA precursors through the activation of mitogen-activated protein kinase PMID: 16895582
11. Depending on the brain's expressions of specific GDNF family GFRalpha2 receptor spliced isoforms, neurturin may promote or inhibit neurite outgrowth through the multicomponent receptor complex. PMID: 17522305
12. GDNF, NTN, GFRalpha-1, GFRalpha-2, and c-Ret proteins are differentially expressed in the different stages of hair follicle cycle. GFRalpha-mediated signaling involves c-Ret and may play a role in human HF biology. PMID: 18222320
13. No differences were found in the allelic frequencies of the variants or in the haplotype distribution between Hirschsprung's disease patients & controls, nor to any demographic/clinical parameters within the group of patients. PMID: 18970938
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相關疾病：

Genetic variations in NRTN may contribute to Hirschsprung disease, in association with mutations of RET gene, and possibly mutations in other loci. Hirschsprung disease is a disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
亞細胞定位：

Secreted.
蛋白家族：

TGF-beta family, GDNF subfamily
數據庫鏈接：

HGNC: 8007

OMIM: 602018

KEGG: hsa:4902

STRING: 9606.ENSP00000302648

UniGene: Hs.234775

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.