Human Neuron-specific enolase,NSE ELISA Kit
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中文名稱：
人神經特異性烯醇化酶(NSE)酶聯免疫試劑盒
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貨號：
CSB-E07961h
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規格：
96T/48T
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價格：
￥3600/￥2500
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其他：
產品詳情
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產品描述：
人神經特異性烯醇化酶(NSE)酶聯免疫試劑盒（CSB-E07961h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ENO2含量。ENO2又名烯醇化酶2，是一種糖酵解酶。它在神經元中高度表達，在神經發育、維持神經功能等方面有重要作用。研究發現，它與神經母細胞瘤等多種腫瘤的發生發展相關，可能通過調節糖代謝和腫瘤微環境等機制影響腫瘤細胞的生長與存活。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，靈敏度為0.195 ng/mL。適用于神經疾病機制研究、腫瘤標志物篩選、藥物作用評估等科研領域，尤其可用于體外模型中神經損傷修復、腫瘤微環境調控等實驗的NSE動態監測，為神經生物學、腫瘤學及轉化醫學研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：
2 phospho D glycerate hydrolyase ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Eno 2 ELISA Kit; ENO2 ELISA Kit; ENOG ELISA Kit; ENOG_HUMAN ELISA Kit; Enolase 2 (gamma; neuronal) ELISA Kit; Enolase 2 ELISA Kit; Enolase 2 gamma neuronal ELISA Kit; Enolase2 ELISA Kit; Epididymis secretory protein Li 279 ELISA Kit; Gamma enolase ELISA Kit; Gamma-enolase ELISA Kit; HEL S 279 ELISA Kit; Neural enolase ELISA Kit; Neuron specific enolase ELISA Kit; Neuron specific gamma enolase ELISA Kit; Neuron-specific enolase ELISA Kit; neuronal enriched enolase ELISA Kit; Neurone specific enolase ELISA Kit; NSE ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, plasma, tissue homogenates
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檢測范圍：
0.78 ng/mL-50 ng/mL
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靈敏度：
0.195 ng/mL
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反應時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領域：
Metabolism
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
84-92 |
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| 1:2 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:4 |
Average % |
99 |
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| Range % |
95-104 |
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| 1:8 |
Average % |
102 |
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| Range % |
96-108 |
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回收率：
| The recovery of human NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
88-96 |
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| EDTA plasma (n=4) |
96 |
90-102 |
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標準曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.341 |
2.332 |
2.337 |
2.240 |
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| 25 |
1.981 |
1.978 |
1.980 |
1.883 |
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| 12.5 |
1.549 |
1.532 |
1.541 |
1.444 |
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| 6.25 |
1.014 |
0.993 |
1.004 |
0.907 |
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| 3.12 |
0.658 |
0.634 |
0.646 |
0.549 |
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| 1.56 |
0.396 |
0.382 |
0.389 |
0.292 |
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| 0.78 |
0.292 |
0.288 |
0.290 |
0.193 |
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| 0 |
0.098 |
0.095 |
0.097 |
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本試劑盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human NSE antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled NSE antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數據處理：
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貨期：
3-5 working days
引用文獻
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Value of Biomarkers in the Prediction of Shunt Responsivity in Patients with Normal Pressure Hydrocephalus
M Cihlo, P Trávní?ek, A Tichá, R Hy?pler, M Kalousová,Neurosurgical Review,2025
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Predictive value of soluble CD59 for poor 28-day neurological prognosis and all-cause mortality in patients after cardiopulmonary resuscitation: a prospective observatory study
L Wang,Journal of intensive care,2023
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Acute effects of ambient nitrogen dioxide exposure on serum biomarkers of nervous system damage in healthy older adults
J Song,Ecotoxicology and environmental safety,2022
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Assessment of Neuron Specific Enolase Level and some Related Biochemical Factors in Patients with Diabetic Peripheral Nerve Disorders.
ZJ Ghaib,Indian Journal of Forensic Medicine & Toxicology,2021
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An ultrasensitive immunosensor based on tri-armed star poly(glycidyl methacrylate) polymer-coated ITO-PET electrode for detection of neuron-specific enolase in human serum
Aydin M, et al,International Journal of Environmental Analytical Chemistry,2019
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Differential long noncoding RNA expressions in peripheral blood mononuclear cells for detection of acute ischemic stroke
Qi-Wen Deng.et al,Clinical Science,2018
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A pilot trial of l-carnitine in patients with traumatic brain injury: Effects on biomarkers of injury
Mahmoodpoor A.et al,J Crit Care,2018
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Serum neuron-specific enolase, biogenic amino-acids and neurobehavioral function in lead-exposed workers from lead-acid battery manufacturing process.
Ravibabu K.et al,Int J Occup Environ Med.,2015
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Synthesis of sunflower-like gold nanostructures and their application in electrochemical immunoassays using the nanogold-triggered hydrogen evolution reaction
Xiaohong Fu. et al,Analytical Methods,2016
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Magnetic Graphene Nanosheet-Based Electrochemical Immunosensing Platform for Sensitive Monitoring of Neuron-Specific Enolase
Li, Guang-Zhou et al,Sensor Letters,2013
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A portable and quantitative enzyme immunoassay of neuron-specific enolase with gluometer readout
Xiaohong Fu et al,Analytical Methods,2014
靶點詳情
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最新研究進展：
ENO2，又稱為神經元特異性烯醇化酶，是一種在神經元中廣泛表達的酶，參與多種神經過程的調節，包括神經元的分化、遷移、突觸形成和記憶等。最新的研究顯示，ENO2的異常表達與神經系統疾病的發生和發展密切相關，例如阿爾茨海默病、帕金森病和多發性硬化癥等。
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功能：
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
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基因功能參考文獻：
- CgA and NSE are clinically valuable tumor markers in neuroblastoma and they merit prospective clinical evaluations as such. PMID: 29737901
- the major result was that elevated serum NSE appears to correlate with prognosis in advanced prostate cancer (PCa), particularly in patients with progressive and metastatic castration-resistant PCa. Based on the existing literature, the role of serum NSE in PCa patients should be further evaluated. PMID: 28885659
- Identify a possible cross-reactive conformational B cell epitope, shared by Borrelia enolase and gamma enolase, but not by alpha enolase. We hypothesize that this autoantibody interferes with glycolysis in neuronal cells, as the physiological basis for chronic symptoms in at least some cases of post-treatment Lyme disease syndrome. PMID: 29317049
- We investigated the possible correlation between TCD results and S100B, NSE and LDH levels as well as between NSE-LDH and S100B-LDH levels by regression analysis. We found a statistically significant relationship for both NSE, S100B with LDH. PMID: 29244749
- ENO2 promoted cell growth, glycolysis, and glucocorticoid resistance, all of which were effectively inhibited when ENO2 was silenced with shRNAs and up-regulated various glycolysis-related genes and enhanced Akt activity with subsequent glycogen synthase kinase3beta (GSK-3beta) phosphorylation, inducing cell proliferation and glycolysis. PMID: 29689546
- High Neuron-Specific Enolase expression is associated with poor response to therapy in small cell lung cancer. PMID: 28127669
- In neonates, NSE and s100B levels increase after bypass surgery and return below preoperative baseline levels by postoperative day seven. PMID: 28571730
- elevated preoperative NSE serum level is adverse predictive and prognostic indicator in adenosquamous lung carcinoma PMID: 27623437
- There is a significant correlation between mortality in the critically ill patients in the intensive care unit and increased serum concentration of S100B and NSE. PMID: 29222857
- The detection of TK1 combined with that of cytokeratin-19 fragment (CYFRA21-1), CEA or NSE increased the diagnostic value of TK1 for lung squamous cell carcinoma, adenocarcinoma and small cell lung cancer, respectively. PMID: 29247745
- Serum levels of neuron-specific enolase (and S100B) are both significantly increased postoperatively and predict postoperative cognitive dysfunction. PMID: 27433840
- This study indicates that serum neuron-specific enolase is elevated in diabetic ketoacidosis and correlated with the severity of hyperglycemia, ketosis, and acidosis. PMID: 28056586
- serum level elevated in small cell lung cancer patients with shorter progression-free survival PMID: 29145241
- Levels of NSE and S100B are still elevated 1 year postpartum in women who have had preeclampsia in contrast to women with previous normal pregnancies. PMID: 27653032
- High NSE expression is associated with hypoxic-ischemic encephalopathy. PMID: 28931055
- we conducted a retrospective study to evaluate the correlation between clinical-pathological parameters, serum levels of neuron-specific enolase and CYFRA21-1, and bone metastases in lung cancer patients.Multivariate logistic regression analysis showed that patients' age concentrations of neuron-specific enolase and histopathological types were the independent risk factors for bone metastases in patients with lung cancer PMID: 28671048
- Suggest that NSE may be a practical parameter that can be used to monitor TB activity and treatment response. Elevated serum NSE level originates, at least in part, from macrophages in granulomatous lesions. PMID: 27271274
- HER2 and NSE serum levels were independent prognostic factors in patients with brain metastases in breast cancer. Serum HER2 and MMP-9 appear to be associated with BM in metastatic BC patients. Their predictive value for BM still needs to be evaluated in further prospective studies. PMID: 27464303
- this study shows that neuron-specific enolase level is both a predictive marker of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors treatment and a prognostic marker in non-small cell lung cancer harboring EGFR mutations patients PMID: 26719155
- The study aimed to identify a small set of genetic signatures that may reliably predict the individuals with a high genetic propensity to heroin addiction. A set of 4 genes (JUN, CEBPB, PRKCB, ENO2, or CEBPG) could predict the diagnosis of heroin addiction with the accuracy rate around 85% in our dataset. PMID: 27495086
- High neuron-specific enolase expression is associated with poor response to chemotherapy in small cell lung cancer. PMID: 26886220
- In pregnant women developing preeclampsia, the levels of NSE remained high throughout pregnancy, compared to healthy women where the levels decline. PMID: 26355117
- Serum neuron specific enolase was a useful marker in predicting subclinical brain damage following essential hypertension. PMID: 27000243
- BALF and serum NSE, CEA, and CYFRA21.1 elevated in lung cancer, which had prompt value for pathology, especially significant for BALF. PMID: 27072263
- Significant levels of CEA, CYFRA 21-1, NSE, and TSGF were detected in the serum. The amounts found were useful for diagnosing non-small cell lung cancer (NSCLC) patients who depended on the currently limited biomarker development. PMID: 27072222
- higher serum and nasal secretion levels were measured in patients with chronic rhinosinusitis compared with healthy controls PMID: 26867533
- CEA, NSE, CA125 and pro-GRP could serve as biomarkers for SCLC, and CEA and CYFRA21-1 could serve as biomarkers for NSCLC. Pro-GRP, CA125 and CEA were related to the clinical stages of lung cancer PMID: 26560853
- Serum neuron-specific enolase level is an independent predictor of overall survival in patients with gastroenteropancreatic neuroendocrine tumors PMID: 26712902
- Vimentin, Nestin and WT1. Sox2 was expressed by the stem/progenitor cells of the ventricular zone, whereas the postmitotic neurons of the cortical plate were immunostained by PAX2 and NSE. PMID: 26972711
- Elevation of neuron specific enolase and brain iron deposition is associated with beta-propeller protein-associated neurodegeneration. PMID: 26481852
- Data show that serum neuron-specific enolase, cytokeratin 19 fragment 21-1, pro-gastrin-releasing peptide, squamous cell carcinoma antigen, tissue inhibitor of metalloproteinase-1, and human epididymis protein 4 are not associated with brain metastases. PMID: 26730601
- Neuron specific enolase levels were not effective in predicting outcomes or detecting recurrences of Merkel cell carcinoma. PMID: 26299616
- This study inquires into the physiological concentrations of NSE and S100B proteins in CSF, carried out on a sufficiently large group of 601 patients. PMID: 26421286
- NSE values is the best predictors of poor 6-month outcome after out-of-hospital cardiac arrest PMID: 26324761
- Studied diagnostic Value of Serum Levels of GFAP, pNF-H, and NSE Compared With Clinical Findings in Severity Assessment of Human Traumatic Spinal Cord Injury. PMID: 25341992
- The association was studied of intracranial pressure (ICP) and cerebral perfusion pressure (CPP) on S-100B and neuron-specific enolase (NSE) in severe traumatic brain injury (sTBI). PMID: 25518864
- In this study, the appearance of NeuN-positive cells were observed in the basal plate as early as 5 month of gestation. PMID: 25904356
- the present findings emphasize that S100 and NSE-immunopositivity might be useful for detecting the cause and process of death due to traumatic brain injury. PMID: 25211554
- Report ontogenic development of nerve fibers in human fetal livers using immunohistochemical detection of NCAM1/neurone-specific enolase expression. PMID: 25326085
- The use of serum NSE as a diagnostic marker for disability progression in multiple sclerosis was not supported. PMID: 25686504
- Serum neuron specific enolase levels were found significantly increased in acute stroke cases as compared to controls. PMID: 24838548
- Neuron-specific enolase levels were able to diagnose sepsis-associated encephalopathy but with less sensitivity than S100Beta. PMID: 24760429
- results suggested the use of serum S-100beta and NSE levels as indicators for brain injury PMID: 26040109
- High levels of neuron-specific enolase (NSE), carbohydrate antigen 125 (CA125) and squamous cell carcinoma antigen (SCC) were detected in 306 (63.6%), 89 (18.5%) and 125 (26.0%) non-small cell lung cancer patients, respectively. PMID: 23712355
- The role of S100B protein, neuron-specific enolase, and glial fibrillary acidic protein in the evaluation of hypoxic brain injury in acute carbon monoxide poisoning PMID: 24505052
- NSE expression is a viable biomarker for MM and can be a useful reference for the design and adjustment of clinical MM treatment programs. PMID: 24796851
- Serial NSE analysis during the acute period of ischemic stroke is useful for monitoring hemorrhagic transformation and the blood-brain barrier disruption status PMID: 25183561
- Serum NSE levels are closely associated with peripheral neuropathy in patients with diabetes. PMID: 23846809
- This is the first study to suggest blood NFL mRNA as a surrogate marker for early prediction of prediabetic peripheral neuropathy, while NSE mRNA levels may be of no diagnostic value in prediabetic patients. PMID: 24733614
- In patients treated with mild hypothermia after out of hospital cardiac arrest changes in NSE are more suitable than its absolute serum levels for the prediction of poor neurologic outcome. PMID: 23836424
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亞細胞定位：
Cytoplasm. Cell membrane.
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蛋白家族：
Enolase family
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組織特異性：
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
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數據庫鏈接：
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