Human Neurofilament protein H (NF-H) ELISA kit
產(chǎn)品詳情
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產(chǎn)品描述：
人神經(jīng)絲蛋白H(NF-H)酶聯(lián)免疫試劑盒（CSB-E16097h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、腦脊液（CSF）樣本中的NEFH含量。NEFH即神經(jīng)絲蛋白重鏈，是神經(jīng)元細(xì)胞骨架的重要組成部分。它在維持神經(jīng)元形態(tài)、軸突運(yùn)輸?shù)冗^程中發(fā)揮關(guān)鍵作用。研究機(jī)制方面，聚焦于其表達(dá)異常與神經(jīng)系統(tǒng)疾病如阿爾茨海默病、帕金森病等的關(guān)聯(lián)，為疾病診斷和治療提供新思路。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，主要用于科研領(lǐng)域，支持神經(jīng)退行性疾病機(jī)制研究、藥物療效評估及生物標(biāo)志物探索，尤其適用于腦脊液等珍貴樣本中NF - H的動(dòng)態(tài)變化監(jiān)測，為神經(jīng)損傷程度和疾病進(jìn)展提供量化研究工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：
200 kDa neurofilament protein ELISA Kit; CMT2CC ELISA Kit; Nefh ELISA Kit; Neurofilament heavy polypeptide 200kDa ELISA Kit; Neurofilament heavy polypeptide ELISA Kit; Neurofilament triplet H protein ELISA Kit; NF H ELISA Kit; NF-H ELISA Kit; NFH ELISA Kit; NFH_HUMAN ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, plasma, cerebrospinal fluid (CSF)
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檢測范圍：
0.625 ng/mL-40 ng/mL
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靈敏度：
0.156 ng/mL
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反應(yīng)時(shí)間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領(lǐng)域：
Neuroscience
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human NF-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 86-98 | |
| 1:2 | Average % | 97 | |
| Range % | 93-101 | |
| 1:4 | Average % | 93 | |
| Range % | 84-97 | |
| 1:8 | Average % | 103 | |
| Range % | 95-107 | |
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回收率：
| The recovery of Human NF-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 85 | 80-90 | |
| EDTA plasma (n=4) | 94 | 88-98 | |
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標(biāo)準(zhǔn)曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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 | ng/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.513 | 2.614 | 2.564 | 2.396 | | | 20 | 2.017 | 2.115 | 2.066 | 1.898 | | | 10 | 1.442 | 1.343 | 1.393 | 1.225 | | | 5 | 0.887 | 0.864 | 0.876 | 0.708 | | | 2.5 | 0.531 | 0.545 | 0.538 | 0.370 | | | 1.25 | 0.374 | 0.362 | 0.368 | 0.200 | | | 0.625 | 0.276 | 0.284 | 0.280 | 0.112 | | | 0 | 0.167 | 0.168 | 0.168 | | |
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本試劑盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human NF-H antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled NF-H antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理：
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貨期：
3-5 working days
靶點(diǎn)詳情
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功能：
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
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基因功能參考文獻(xiàn)：
- In newborns undergoing cardiac surgery, phosphorylated axonal neurofilament heavy chain was decreased at 0 hours in both the cardiopulmonary bypass and deep hypothermic circulatory arrest groups compared to baseline. PMID: 29945509
- Phosphoneurofilament heavy chain level was higher in ALS patients than in controls PMID: 27538346
- Data suggest that high CSF NfH levels are an early predictor of later brain and spinal cord atrophy in multiple sclerosis patients. PMID: 27207456
- Findings in the dorsolateral prefrontal cortex in schizophrenia provide evidence of altered proteins involved in synaptic function (FABP4), cytoarchitecture organization (NEFH), and circadian molecular clock signaling (CSNK1E), which may be contributing to the cognitive and/or negative symptoms in this disorder. FABP4, CSNK1E and NEFH could become potentially useful biomarkers for schizophrenia. PMID: 27236410
- Unique deletions of two nucleotides causing frameshifts near the end of the NEFH coding sequence were identified in two Charcot-Marie-Tooth disease families. Using electroporation of chick embryo spinal cord, confirmed that NEFH mutants form aggregates in vivo and trigger apoptosis of spinal cord neurons. PMID: 28709447
- This study confirmed the general applicability of the monocentric obtained cut-off values for neurofilamens in ALS, especially for pNfH PMID: 27415180
- data support the use of Cebrospinal fluid phosphorylated NFH as a prognostic biomarker for amyotrophic lateral sclerosis. PMID: 28628244
- Study found a significant correlation between values of 8-hydroxy-2'-deoxyguanosine and phosphorylated NF-H only in clinically isolated syndrome group. While the plasma values of 8-hydroxy-2'-deoxyguanosine reflect the degree of acute demyelination in clinically isolated syndrome, phosphorylated NF-H values reflect that in relapsing-remitting multiple sclerosis. PMID: 27295058
- Results provide evidence that in particular pNfH can be used as a good diagnostic biomarker of ALS at the diagnostic stage. Moreover, results indicate that NfL may be useful in monitoring disease progression in a subset of patients. PMID: 28500227
- Level of neurofilament heavy chain and light chains were significantly elevated in the cerebrospinal fluid of Amyotrophic Lateral Sclerosis (ALS) patients compared to healthy controls/controls without parenchymal central nervous system involvement and ALS mimic disease patients. PMID: 27732645
- Study found a significant increase of pNF-H levels in both plasma and CSF in amyotrophic lateral sclerosis patients PMID: 27423602
- Studied diagnostic Value of Serum Levels of GFAP, pNF-H, and NSE Compared With Clinical Findings in Severity Assessment of Human Traumatic Spinal Cord Injury. PMID: 25341992
- This study demonstrated that Higher NF-L concentrations (beta = -0.26) were associated with functional decline in patients with vascular burden. PMID: 25633679
- Phospho-NFH levels were significantly higher in amyotrophic lateral sclerosis patients in comparison with controls, in particular in fast progressors. PMID: 25261856
- Data identified NEFH methylation as a candidate epigenetic marker for prognosis of RCC patients as well as prediction of anti-vascular endothelial growth factor-based therapy response. PMID: 24464810
- pNFL-H may be useful in determining which individuals require CT imaging to assess the severity of their injury PMID: 25192482
- Subconcussive repetitive trauma in amateur boxing causes a mild traumatic brain injury that may be diagnosed by CSF analysis of expressed pNFH, even without unconsciousness or concussion symptoms. PMID: 24260563
- Absence of axonal neurofilaments in NFH-LacZ transgenic mice compromises axonal regeneration. PMID: 23079625
- In conclusion, pNfH represents a promising candidate for inclusion in a panel of diagnostic and prognostic biomarkers. PMID: 23134506
- CSF and intrathecal levels and CSF/serum ratios of anti-NFH antibodies were increased in the CIS patients early developing multiple sclerosis. PMID: 23632043
- These data support further study of pNF-H in CSF, serum and plasma as a potential amyotrophic lateral sclerosis biomarker PMID: 23117489
- NF-H levels increase significantly faster in children who have a worse Glasgow Outcome Scale following traumatic brain injury, or died. PMID: 21976236
- significant in vivo information on the pathophysiology of stroke may be obtained by the determination of NfH(SMI35). PMID: 21792676
- Serum neurofilament protein H levels were significantly elevated in stroke patients compared to healthy controls. PMID: 21349546
- Data suggest that a deficiency of cellular neuroprotective mechanisms (decrease of sAPP) is linked to progressive neuro-axonal damage (increase of NfH(SMI35)) and to progression of disease. PMID: 21858182
- Data show that RNA interference-mediated knockdown of NEFH accelerated ESCC cell growth in culture and increased tumorigenicity in vivo. PMID: 20140245
- NEFH is phosphorylated at serine 493 by GSK3b PMID: 12130654
- NEFH gene is involved in the pathogenesis of sporadic motor neuron disease PMID: 14722583
- mutations in neurofilaments are possible risk factors that may contribute to pathogenesis in amyotrophic lateral sclerosis in conjunction with one or more additional genetic or environmental factors, but are not significant primary causes PMID: 16084104
- A subgroup of FTD patients had remarkably high CSF levels of NfH. The degree of NfH phosphorylation was increased in FTD compared to both other groups. PMID: 17290105
- Isomerization of lys-ser-pro repeat residues that are abundant in NF-H tail domains by Pin1 can regulate NF-H phosphorylation. PMID: 17626162
- analysis of major neurofilament subunit NF-H levels in blood and cerebrospinal fluid differentiates between patients with poor and favorable outcomes. PMID: 18319731
- Pin1 as a possible modulator of stress-induced NF-H phosphorylation as seen in neurodegenerative disorders like AD and amyotrophic lateral sclerosis PMID: 18635547
- Elevated pNF-H released into the serum of some affected Leber hereditary optic neuropathy patients may suggest that axonal degeneration occurs at some point after loss of visual function. PMID: 19104679
- cerebrospinal fluid phosphorylated forms of neurofilament heavy subunit are not molecular markers of axonal damage for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) probably due to the slow progression of this disease. PMID: 19678766
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相關(guān)疾病：
Amyotrophic lateral sclerosis (ALS); Charcot-Marie-Tooth disease 2CC (CMT2CC)
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亞細(xì)胞定位：
Cytoplasm, cytoskeleton. Cell projection, axon.
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蛋白家族：
Intermediate filament family
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數(shù)據(jù)庫鏈接：
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