Human NGAL/MMP-9 complex ELISA kit

產品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人NGAL/MMP-9復合物酶聯免疫試劑盒（CSB-EQ01026975HU）檢測血清、血漿、唾液、組織培養上清液、組織勻漿樣本中的NGAL/MMP-9 complex含量。NGAL/MMP-9 complex 作為靶點備受關注。它在多種疾病如腫瘤、炎癥等病理過程中發揮重要作用。研究機制上，二者形成復合物后可影響細胞生長、遷移、侵襲等行為，調節組織的微環境，通過干擾其相互作用有望為相關疾病治療提供新思路。試劑盒檢測范圍為0.313 ng/mL-20 ng/mL，靈敏度為0.029 ng/mL。適用于基礎醫學研究中疾病機制探索、生物標志物分析及藥物干預效果評估。為炎癥相關疾病模型構建、腫瘤微環境研究及腎臟功能損傷實驗提供高效工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

NGAL/MMP-9 complex
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, saliva, cell culture supernates, tissue homogenates
檢測范圍：

0.313 ng/mL-20 ng/mL
靈敏度：

0.029 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human NGAL/MMP-9 complex in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	91
Range %	87-98
1:20	Average %	95
Range %	90-102
1:40	Average %	93
Range %	87-99
1:80	Average %	94
Range %	86-105

回收率：

The recovery of human NGAL/MMP-9 complex spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-103
EDTA plasma (n=4)	94	87-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.