Human N-terminal procollagen Ⅳ propeptide,PⅣNP ELISA Kit

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產品詳情

產品描述：

人Ⅳ型前膠原氨基末端肽(PⅣNP)酶聯免疫試劑盒（CSB-EQ027190HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PⅣNP含量。試劑盒檢測范圍為2.8 ng/mL-180 ng/mL，常用于肝纖維化、肺纖維化等疾病模型的機制研究，適用于體外培養體系、動物實驗樣本中膠原合成活性的動態監測，可為纖維化相關分子機制探索、藥物干預效果評估等研究提供可靠數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N-terminal procollagen 4 propeptide,P4NP,_x000D_ N-terminal procollagen IV propeptide,PIVNP
縮寫：

PⅣNP
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

2.8 ng/mL-180 ng/mL
靈敏度：

0.7 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PIVNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	99
	Range %	87-104
1:10	Average %	105
	Range %	100-110
1:20	Average %	93
	Range %	86-97
1:40	Average %	104
	Range %	95-107

回收率：

The recovery of human PIVNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	107	100-111
EDTA plasma (n=4)	101	95-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.