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中文名稱：人粘蛋白-15(MUC15)酶聯免疫試劑盒
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貨號：CSB-EL015218HU
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規格：96T/48T
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價格：￥3600/￥2500
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其他：
產品詳情
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產品描述：人粘蛋白-15(MUC15)酶聯免疫試劑盒（CSB-EL015218HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的MUC15含量。MUC15屬于黏蛋白家族成員，在多種組織中表達。它參與細胞黏附、信號傳導等過程。研究發現其異常表達與腫瘤發生、發展相關，可能通過調節腫瘤細胞增殖、侵襲和轉移發揮作用，有望成為腫瘤治療的新靶點。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，該產品適用于科研領域對MUC15蛋白表達水平的定量分析，例如在癌癥發生機制、炎癥相關通路研究或組織特異性表達譜分析中評估生物樣本中MUC15的動態變化，為探索其在細胞通訊、疾病生物標志物篩選等基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：Component II ELISA Kit; Glycoprotein 4 ELISA Kit; Glycoprotein C ELISA Kit; MUC 15 ELISA Kit; MUC-15 ELISA Kit; Muc15 ELISA Kit; MUC15_HUMAN ELISA Kit; Mucin 15 ELISA Kit; Mucin 15 cell surface associated ELISA Kit; Mucin-15 ELISA Kit; PAS3 ELISA Kit; PASIII ELISA Kit
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縮寫：MUC15
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, tissue homogenates, cell lysates
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檢測范圍：125 pg/mL-8000 pg/mL
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靈敏度：31.25 pg/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Signal Transduction
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human MUC15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 97 Range % 89-103 1:2 Average % 95 Range % 90-99 1:4 Average % 97 Range % 87-102 1:8 Average % 103 Range % 97-108 -
回收率：
The recovery of human MUC15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 86 81-90 EDTA plasma (n=4) 92 88-98 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 8000 1.972 2.027 2.000 1.863 4000 1.456 1.436 1.446 1.309 2000 0.902 0.925 0.914 0.777 1000 0.576 0.583 0.580 0.443 500 0.430 0.421 0.426 0.289 250 0.317 0.309 0.313 0.176 125 0.250 0.242 0.246 0.109 0 0.140 0.134 0.137 -
數據處理：
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貨期：3-5 working days
引用文獻
- Ascitic Shear Stress Activates GPCRs and Downregulates Mucin 15 to Promote Ovarian Cancer Malignancy G Mehta, E Horst, L Cotter, M Bonini, C Novak,Research Square,2024
產品評價
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靶點詳情
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功能：May play a role in the cell adhesion to the extracellular matrix.
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基因功能參考文獻：
- Frameshift mutations of MUC15 gene is associated with gastric and colorectal cancers. PMID: 25573589
- Multivariate analysis suggested that MUC15 overexpression was an independent factor for prognosis (hazard risk: 3.216; P=0.009). It was concluded that MUC15 is overexpressed in glioma tissues. PMID: 24710941
- MUC15 inhibits dimerization of EGFR and PI3K-AKT signaling and is associated with aggressive hepatocellular carcinomas in patients. PMID: 23933603
- MUC15 is a strong candidate for eczema. PMID: 22657408
- MUC4 and MUC15 were overexpressed in papillary thyroid carcinoma, and high MUC15 expression was associated with high malignant potential. PMID: 21615302
- MUC15 was expressed most highly in human placenta. MUC15 mRNA & protein increased with gestational age (P < 0.05, 1st versus 3rd trimester). Differential expression of MUC15 in human placentas may play critical role in regulation of trophoblast invasion. PMID: 17720698
- Overexpression of MUC15 activates extracellular signal-regulated kinase 1/2 and promotes the oncogenic potential of colon cancer cells. PMID: 19520792
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亞細胞定位：[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
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組織特異性：Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, bone marrow, lymph node and lung.
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數據庫鏈接：
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