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中文名稱：人黑色素瘤相關抗原D2(MAGED2)酶聯免疫試劑盒
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貨號：CSB-EL013352HU
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規格：96T/48T
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價格：￥3600/￥2500
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其他：
產品詳情
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產品描述：人黑色素瘤相關抗原D2(MAGED2)酶聯免疫試劑盒（CSB-EL013352HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的MAGED2含量。MAGED2基因是MAGE家族成員之一，位于X染色體上，與X連鎖智力障礙和某些癌癥相關。其蛋白在細胞周期調節中發揮作用，可變剪接導致多個轉錄本變體。研究發現，MAGED2蛋白具有抗病毒功能，新冠病毒主蛋白酶可切割MAGED2蛋白，使其失去抗病毒作用。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，本產品可廣泛應用于腫瘤分子機制研究、抗癌藥物體外篩選及腫瘤相關生物標志物的表達水平分析等科研領域，為探索MAGED2在腫瘤微環境調控、轉移潛能評估等方向提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：11B6 ELISA Kit; BCG 1 ELISA Kit; BCG-1 ELISA Kit; Breast cancer-associated gene 1 protein ELISA Kit; HCA10 ELISA Kit; Hepatocellular carcinoma-associated protein JCL-1 ELISA Kit; JCL 1 ELISA Kit; MAGD2_HUMAN ELISA Kit; MAGE D ELISA Kit; MAGE D2 antigen ELISA Kit; MAGE-D2 antigen ELISA Kit; MAGED2 ELISA Kit; Melanoma antigen family D 2 ELISA Kit; Melanoma associated antigen D2 ELISA Kit; Melanoma-associated antigen D2 ELISA Kit
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縮寫：MAGED2
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, tissue homogenates, cell lysates
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檢測范圍：31.25 pg/mL-2000 pg/mL
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靈敏度：7.81 pg/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Cancer
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human MAGED2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 89 Range % 83-94 1:2 Average % 97 Range % 92-101 1:4 Average % 93 Range % 88-97 1:8 Average % 107 Range % 102-111 -
回收率：
The recovery of human MAGED2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 105 102-108 EDTA plasma (n=4) 96 91-98 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 2000 2.238 2.334 2.286 2.090 1000 1.415 1.527 1.471 1.275 500 0.881 0.932 0.907 0.711 250 0.560 0.573 0.567 0.371 125 0.379 0.381 0.380 0.184 62.5 0.292 0.284 0.288 0.092 31.25 0.247 0.252 0.250 0.054 0 0.195 0.196 0.196 -
數據處理：
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貨期：3-5 working days
引用文獻
- Serum levels of ANOS1 serve as a diagnostic biomarker of gastric cancer: a prospective multicenter observational study Kanda M, et al,Gastric Cancer,2019
- The Expression of Melanoma-Associated Antigen D2 Both in Surgically Resected and Serum Samples Serves as Clinically Relevant Biomarker of Gastric Cancer Progression Mitsuro Kanda MD, PhD et al,Annals of Surgical Oncology,2015
產品評價
相關產品
靶點詳情
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功能：Regulates the expression, localization to the plasma membrane and function of the sodium chloride cotransporters SLC12A1 and SLC12A3, two key components of salt reabsorption in the distal renal tubule.
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基因功能參考文獻：
- Increased expression of MAGE-D2 mRNA was associated with distant metastasis in Gastric Cancer. PMID: 25743330
- MAGE-D2 is a dynamic protein whose shuttling properties could suggest a role in cell cycle regulation. PMID: 26705694
- We found that MAGED2 mutations caused X-linked polyhydramnios with prematurity and a severe but transient form of antenatal Bartter's syndrome. PMID: 27120771
- these results identify the expression of MAGE-D2 suppresses TRAIL receptor 2 and protects againstas TRAIL-induced apoptosis PMID: 22791814
- Expression pattern and further characterization of human MAGED2 and identification of rodent orthologues PMID: 11856887
- Maged2 is mainly expressed in tissues of mesodermal origin PMID: 15162511
- Results identified a cDNA clone, corresponding to MAGE D2 mRNA, from primary human bronchial epithelial cells which exhibits increased expression in vitro after treatment with all-trans retinoic acid. PMID: 15465002
- MAGED2, a novel protein, is a p53-dissociator. PMID: 17912449
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相關疾病：Bartter syndrome 5, antenatal, transient (BARTS5)
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組織特異性：Widely expressed. In the developing and adult kidney, expressed in the thick ascending limb of the loop of Henle and the distal convoluted tubules outside the loop.
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數據庫鏈接：
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