Human Matrix-remodeling-associated protein 8(MXRA8) ELISA kit

文獻引用 (1) 說明書下載

中文名稱：

人基質重塑關聯蛋白8(MXRA8)酶聯免疫試劑盒
貨號：

CSB-EL015257HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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人基質重塑關聯蛋白8(MXRA8)酶聯免疫試劑盒（CSB-EL015257HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MXRA8含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，支持科研人員開展疾病模型（如纖維化疾病、病毒感染或腫瘤微環境等）中基質重塑相關機制研究；可用于評估不同處理條件下組織樣本中MXRA8的動態變化；適用于基礎醫學、分子生物學及病理學領域的科學研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Limitrin ELISA Kit; Matrix remodelling associated 8 ELISA Kit; Matrix-remodeling-associated protein 8 ELISA Kit; Mxra8 ELISA Kit; MXRA8_HUMAN ELISA Kit
縮寫：

MXRA8
Uniprot No.：

Q9BRK3
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MXRA8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-92
1:2	Average %	109
	Range %	101-113
1:4	Average %	92
	Range %	85-97
1:8	Average %	91
	Range %	83-95

回收率：

The recovery of human MXRA8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	82-94
EDTA plasma (n=4)	103	94-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.306	2.217	2.262	2.150
5	1.543	1.523	1.533	1.421
2.5	0.952	1.001	0.977	0.865
1.25	0.619	0.600	0.610	0.498
0.625	0.363	0.343	0.353	0.241
0.312	0.259	0.252	0.256	0.144
0.156	0.174	0.181	0.178	0.066
0	0.110	0.114	0.112

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Protective Role of Limitrin in Experimental Autoimmune Optic Neuritis BY Chun,Investigative Ophthalmology & Visual Science,2021

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功能：

Transmembrane protein which can modulate activity of various signaling pathways, probably via binding to integrin ITGAV:ITGB3. Mediates heterophilic cell-cell interactions in vitro. Inhibits osteoclastogenesis downstream of TNFSF11/RANKL and CSF1, where it may function by attenuating signaling via integrin ITGB3 and MAP kinase p38. Plays a role in cartilage formation where it promotes proliferation and maturation of growth plate chondrocytes. Stimulates formation of primary cilia in chondrocytes. Enhances expression of genes involved in the hedgehog signaling pathway in chondrocytes, including the hedgehog signaling molecule IHH; may also promote signaling via the PTHLH/PTHrP pathway. Plays a role in angiogenesis where it suppresses migration of endothelial cells and also promotes their apoptosis. Inhibits VEGF-induced activation of AKT and p38 MAP kinase in endothelial cells. Also inhibits VTN (vitronectin)-mediated integrin ITGAV:ITGB3 signaling and activation of PTK2/FAK. May play a role in the maturation and maintenance of the blood-brain barrier.; (Microbial infection) Contributes to arthritogenic alphavirus pathogenesis and acts as a receptor for these viruses.
基因功能參考文獻：
1. exogenous expression of DICAM in human umbilical vein endothelial cells suppressed angiogenesis in vitro Matrigel and in vivo plug assays, and conversely, DICAM knockdown enhanced angiogenesis PMID: 23386276
2. MXRA8 is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
3. new member of the transmembrane-type immunoglobulin superfamily and designated limitrin, localizing selectively to glia limitans in mouse brain PMID: 14603461
亞細胞定位：

Cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Cytoplasm. Cell projection, cilium membrane. Nucleus.
組織特異性：

Detected in endothelial cells in mammary tissue, in both large vessels (left internal mammary artery) and small capillaries (vasa vasorum of the adventitia).
數據庫鏈接：

HGNC: 7542

OMIM: 617293

KEGG: hsa:54587

STRING: 9606.ENSP00000307887

UniGene: Hs.515687

Human Matrix-remodeling-associated protein 8(MXRA8) ELISA kit

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