Human Mast Cell Carboxypeptidase ELISA Kit

Human Mast Cell Carboxypeptidase ELISA Kit

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中文名稱：

人肥大細胞類羧肽酶酶聯免疫試劑盒
貨號：

CSB-E13756h
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人肥大細胞類羧肽酶酶聯免疫試劑盒（CSB-E13756h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CPA3含量。CPA3是一個值得關注的靶點。它在人體生理與病理過程中發揮作用。目前研究聚焦于其參與的免疫反應、炎癥調節等機制。了解其作用機制有助于深入認識相關疾病發病原理，為開發針對性治療手段如藥物研發等提供重要方向。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于科研領域中對肥大細胞相關疾病機制的研究，如過敏性疾病模型構建、炎癥標志物分析以及藥物干預下CPA3表達水平變化的評估，為探索肥大細胞功能調控提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

carboxypeptidase A ELISA Kit; carboxypeptidase A3 (mast cell) ELISA Kit; Carboxypeptidase A3 ELISA Kit; carboxypeptidase A3 mast cell ELISA Kit; CBPA3_HUMAN ELISA Kit; Cpa3 ELISA Kit; Mast cell carboxypeptidase A ELISA Kit; mast cell carboxypeptidase A3 ELISA Kit; MC CPA ELISA Kit; MC-CPA ELISA Kit; tissue carboxypeptidase A ELISA Kit
縮寫：

CPA3
Uniprot No.：

P15088
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MC-CPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	100
	Range %	94-103
1:4	Average %	97
	Range %	94-100
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of human MC-CPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	96	94-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.477	2.453	2.465	2.366
10	1.993	1.924	1.959	1.860
5	1.280	1.271	1.276	1.177
2.5	0.685	0.678	0.682	0.583
1.25	0.433	0.424	0.429	0.330
0.625	0.286	0.269	0.278	0.179
0.312	0.190	0.179	0.185	0.086
0	0.101	0.097	0.099

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

基因功能參考文獻：
1. Carboxypeptidase A3 was differentially expressed between Fibromyalgia patients and healthy controls. PMID: 27157394
2. Urine CPA3 was increased in patients with chronic prostatitis/chronic pelvic pain syndrome compared to controls. PMID: 24726923
3. The cDNA encoding human colon mast cell carboxypeptidase can be successfully cloned and expressed in E. coli and P. pastoris. PMID: 14760754
4. Role in regulating innate immunity responses and regulating secretory granule homeostasis PMID: 19643669
亞細胞定位：

Cytoplasmic vesicle, secretory vesicle. Note=Secretory granules.
蛋白家族：

Peptidase M14 family
數據庫鏈接：

HGNC: 2298

OMIM: 114851

KEGG: hsa:1359

STRING: 9606.ENSP00000296046

UniGene: Hs.646

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.