Human Loricrin(LOR) ELISA kit

Human Loricrin(LOR) ELISA kit

產品詳情

引用文獻

產品評價

靶點詳情

產品詳情

引用文獻

產品評價

相關產品

靶點詳情

Most popular with customers

產品中心

信號通路

技術信息

聯系信息

文獻引用 (1) 說明書下載

中文名稱：

人兜甲蛋白(LOR)酶聯免疫試劑盒
貨號：

CSB-EL013037HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產品詳情
產品評價
引用文獻
相關產品
靶點詳情

產品描述：

人兜甲蛋白(LOR)酶聯免疫試劑盒（CSB-EL013037HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的LOR含量。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，適用于科研場景中皮膚生物學機制研究、疾病模型表皮分化評估或藥物干預效果分析。科研人員可通過本產品定量檢測不同實驗條件下LOR的表達水平變化，為探索皮膚屏障功能調控、表皮異常分化機制及相關疾病發病機理提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

LOR ELISA Kit; LOR protein ELISA Kit; LORI_HUMAN ELISA Kit; Loricrin ELISA Kit; LRN ELISA Kit; MGC111513 ELISA Kit; OTTHUMP00000015823 ELISA Kit
縮寫：

LOR
Uniprot No.：

P23490
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

25 pg/mL-1600 pg/mL
靈敏度：

6.25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human LOR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	97
	Range %	94-100
1:4	Average %	98
	Range %	95-101
1:8	Average %	87
	Range %	82-91

回收率：

The recovery of human LOR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	102	98-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.424	2.468	2.446	2.300
800	1.561	1.598	1.580	1.434
400	0.986	0.946	0.966	0.820
200	0.634	0.657	0.646	0.500
100	0.411	0.435	0.423	0.277
50	0.295	0.275	0.285	0.139
25	0.202	0.213	0.208	0.062
0	0.145	0.147	0.146

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

A lipid mixture enriched by ceramide NP with fatty acids of diverse chain lengths contributes to restore the skin barrier function impaired by topical corticosteroid SH Lim,Skin pharmacology and physiology,2023

功能：

Major keratinocyte cell envelope protein.
基因功能參考文獻：
1. Authors report a multi-generation family with prominent ichthyosis and palmoplantar involvement due to a novel mutation in loricrin. PMID: 25965869
2. Letter: Knockdown of either filaggrin or loricrin increases the productions of interleukin (IL)-1alpha, IL-8, IL-18 and granulocyte macrophage colony-stimulating factor in stratified human keratinocytes. PMID: 26381575
3. Studies on human keratinocytes recognized that loricrin expression was inversely related to the expression of the cyclin-dependent kinase inhibitor p21 PMID: 25896246
4. two novel heterozygous frameshift mutations in exon 2 - c.646_647insGCAGCAGGTC, p.Gln216Argfs*123 and c.798_799dupT, p.Gly267Trpfs*69 in loricrin keratoderma patients PMID: 25234742
5. Results describe a novel frameshift mutation leading to loricrin keratoderma presenting with colloidion membrane PMID: 25142840
6. We found no mutations of Loricrin in two Progressive symmetrical erythrokeratoderma families. PMID: 23678955
7. We describe a young man who was a collodion baby and had the typical presentation of Loricrin keratoderma. Direct DNA sequencing identified a heterozygous mutation in the loricrin gene with a single G insertion, 730insG, present in (a) the patient PMID: 22831754
8. There were no mutations found in the LOR gene and the true pathogenesis of progressive symmetrical erythrokeratodermia remains unknown. PMID: 21198793
9. These findings suggest that inverse effects of PKCdelta and PKCeta on loricrin expression attributes to the expression of c-Jun and JunD. PMID: 20184865
10. regulation of gene expression requires interactions among multiple transcription factors in keratinocytes located in different compartments of the epidermis PMID: 12200429
11. Unique mutations in glycine-rich domain of mutant loricrin form arginine-rich nuclear localization sequences that disrupt differentiation of keratinocytes. PMID: 12615358
12. Barrier abnormality in loricrin keratoderma is linked to defective CE scaffold, resulting in increased extracellular permeability. PMID: 15102081
13. ruled out as a candidate for the PSORS4 locus. PMID: 15598222
14. report the clinical and molecular characterization of a new family with the recurrent 730insG LOR mutation, giving new insights in LK genotype-phenotype correlation PMID: 17953701
15. results give evidence that heterogeneous phenotypes of LK may be the result of genetic heterogeneity of loricrin mutations, and demonstrate that nuclear accumulation of mutant loricrin is due to the nuclear targeting sequences in the mutant C-terminus. PMID: 18844868
16. the deregulated increase in SPRR1A expression in chronic atopic skin lesions reflects an insufficient rise in SPRR transcripts, unable to compensate for the lack of LOR and thus contributing to the persistence of chronic atopic dermatitis skin lesions. PMID: 19672094
顯示更多

收起更多
相關疾病：

Vohwinkel syndrome with ichthyosis (VSI)
亞細胞定位：

Cytoplasm. Nucleus, nucleoplasm.
數據庫鏈接：

HGNC: 6663

OMIM: 152445

KEGG: hsa:4014

STRING: 9606.ENSP00000357731

UniGene: Hs.251680

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.