Human Lipoxin A4,LXA4 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人脂氧素A4(LXA4)酶聯(lián)免疫試劑盒（CSB-E09689h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、尿液、組織勻漿、細(xì)胞裂解物樣本中的LXA4含量。LXA4（脂氧素A4）是一種內(nèi)源性抗炎和免疫調(diào)節(jié)介質(zhì)，由花生四烯酸通過脂氧合酶作用合成。它通過抑制炎癥細(xì)胞、調(diào)節(jié)促炎和抗炎因子平衡，限制炎癥損傷，在多種生理和病理過程中發(fā)揮重要作用。試劑盒檢測范圍為0.625 pg/mL-40 pg/mL，該產(chǎn)品可用于體外研究炎癥相關(guān)信號通路、評估細(xì)胞模型或動物模型中LXA4的動態(tài)變化，或探索其在組織微環(huán)境調(diào)控中的作用，為炎癥性疾病機(jī)制研究提供工具支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

LXA4
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, urine, tissue homogenates, cell lysates
檢測范圍：

0.625 pg/mL-40 pg/mL
靈敏度：

0.156 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human LXA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
	Range %	84-92
1:200	Average %	94
	Range %	90-98
1:400	Average %	99
	Range %	95-104
1:800	Average %	95
	Range %	88-99

回收率：

The recovery of human LXA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-99
EDTA plasma (n=4)	100	95-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.