Human Layilin(LAYN) ELISA kit

Human Layilin(LAYN) ELISA kit

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中文名稱：

人排卵蛋白(LAYN)酶聯(lián)免疫試劑盒
貨號：

CSB-EL012772HU
規(guī)格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產(chǎn)品描述：

人排卵蛋白(LAYN)酶聯(lián)免疫試劑盒（CSB-EL012772HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LAYN含量。人排卵蛋白(LAYN)是一種調(diào)節(jié)T細(xì)胞功能的關(guān)鍵基因，其高表達(dá)與多種癌癥的預(yù)后不良相關(guān)，尤其是與胃癌和結(jié)腸癌的免疫浸潤水平密切相關(guān)。LAYN可能通過調(diào)節(jié)腫瘤相關(guān)巨噬細(xì)胞、DC、T細(xì)胞耗竭和Treg等免疫細(xì)胞在腫瘤微環(huán)境中的功能，影響腫瘤的進(jìn)展和預(yù)后。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，靈敏度為3.9 pg/mL。適用于體外研究LAYN在生殖生理調(diào)控、排卵障礙模型構(gòu)建、卵巢功能評估以及免疫細(xì)胞相互作用等領(lǐng)域的表達(dá)特征，也可用于篩選調(diào)控LAYN表達(dá)的生物活性物質(zhì)或藥物分子，為生殖醫(yī)學(xué)和免疫學(xué)基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Layilin ELISA Kit; LAYN ELISA Kit; LAYN_HUMAN ELISA Kit; UNQ208/PRO234 ELISA Kit
縮寫：

LAYN
Uniprot No.：

Q6UX15
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human LAYN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-86
1:2	Average %	92
	Range %	89-96
1:4	Average %	104
	Range %	101-107
1:8	Average %	89
	Range %	85-93

回收率：

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

2.321

2.341

2.331

2.159

500

1.929

1.902

1.916

1.744

250

1.395

1.376

1.386

1.214

125

0.875

0.841

0.858

0.686

62.5

0.574

0.592

0.583

0.411

31.25

0.396

0.387

0.392

0.220

15.6

0.287

0.291

0.289

0.117

0.171

0.173

0.172

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Receptor for hyaluronate.
基因功能參考文獻(xiàn)：
1. Renal biopsy samples from patients with glomerulonephritis showed high expression of LAYN in tubular epithelial cells. PMID: 26410531
2. results indicate that LAYN would be involved in the enhancement of inflammation and degradation of cartilage in joint diseases such as RA and OA PMID: 25150153
3. Hyaluronan and layilin mediate loss of airway epithelial barrier function induced by cigarette smoke by decreasing E-cadherin. PMID: 23048036
亞細(xì)胞定位：

Membrane; Single-pass type I membrane protein.
數(shù)據(jù)庫鏈接：

HGNC: 29471

KEGG: hsa:143903

STRING: 9606.ENSP00000364764

UniGene: Hs.503831

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.