Human Ketohexokinase(KHK) ELISA kit

Human Ketohexokinase(KHK) ELISA kit

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中文名稱：

人己酮糖激酶(KHK)酶聯免疫試劑盒
貨號：

CSB-EL012157HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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人己酮糖激酶(KHK)酶聯免疫試劑盒（CSB-EL012157HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的KHK含量。KHK即己酮糖激酶，是催化果糖代謝第一步反應的關鍵酶。研究顯示其與代謝綜合征等多種疾病密切相關。其研究機制主要聚焦于KHK對果糖代謝通路的調控，以及通過影響肝臟脂質合成等途徑，在疾病發生發展中所起的作用。試劑盒檢測范圍為18.75 mU/mL-1200 mU/mL，適用于細胞能量代謝研究、動物模型代謝評估及體外藥物干預實驗等科研領域，可為果糖代謝相關分子機制探索、代謝綜合征病理研究及潛在治療靶點篩選提供可靠數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

EC 2.7.1.3 ELISA Kit; Fructokinase ELISA Kit; Hepatic fructokinase ELISA Kit; Ketohexokinase ELISA Kit; Ketohexokinase isoform a ELISA Kit; KHK ELISA Kit; KHK_HUMAN ELISA Kit
縮寫：

KHK
Uniprot No.：

P50053
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

18.75 mU/mL-1200 mU/mL
靈敏度：

4.68 mU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human KHK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-94
1:2	Average %	84
	Range %	80-88
1:4	Average %	95
	Range %	90-99
1:8	Average %	99
	Range %	93-103

回收率：

The recovery of human KHK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-102
EDTA plasma (n=4)	90	86-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
1200	2.547	2.461	2.504	2.352
600	2.143	2.076	2.110	1.958
300	1.403	1.449	1.426	1.274
150	0.814	0.765	0.790	0.638
75	0.304	0.325	0.315	0.163
37.5	0.226	0.218	0.222	0.070
18.75	0.182	0.169	0.176	0.024
0	0.156	0.147	0.152

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.
基因功能參考文獻：
1. Data indicate metabolic enzymes NAD kinase and ketohexokinase as candidate metabolic gene targets, and the chromatin remodeling protein INO80C as a tumor suppressor in KRAS(MUT) colorectal tumor xenograft. PMID: 28954733
2. Angelica archangelica, Garcinia mangostana, Petroselinum crispum, and Scutellaria baicalensis were the top four botanical candidiates identified with inhibitory activity against ketohexokinase-C. PMID: 27322374
3. compared with normal hepatocytes, hepatocellular carcinoma (HCC) cells markedly reduce the rate of fructose metabolism and the level of reactive oxygen species, as a result of a c-Myc-dependent and heterogeneous nuclear ribonucleoprotein (hnRNP) H1- and H2-mediated switch from expression of the high-activity fructokinase (KHK)-C to the low-activity KHK-A isoform. PMID: 27088854
4. myocardial hypoxia actuates fructose metabolism in human and mouse models of pathological cardiac hypertrophy through hypoxia-inducible factor 1alpha (HIF1alpha) activation of SF3B1 and SF3B1-mediated splice switching of KHK-A to KHK-C PMID: 26083752
5. These studies identify fructokinase as a novel mediator of diabetic nephropathy and document a novel role for endogenous fructose production, or fructoneogenesis, in driving renal disease. PMID: 24876114
6. This study determined if single nucleotide polymorphisms in genes involved in fructose transport,SLC2A2 and SLC2A5 and metabolism, etohexokinase affect inter-individual variability in metabolic phenotypes. PMID: 23341889
7. In human hepatocytes uric acid up-regulates KHK expression thus leading to the amplification of the lipogenic effects of fructose. PMID: 23112875
8. ketohexokinase-A serves an unknown physiologic function that remains intact in essential fructosuria. PMID: 12941785
9. The expression of ketohexokinase is diminished in human clear cell type of renal cell carcinoma PMID: 16372272
10. Ketohexokinase-dependent metabolism of fructose induces proinflammatory mediators in proximal tubular cells. PMID: 19158351
11. The structure of the KHK-A ternary complex revealed an active site with fructose & the ATP analogue in positions ready for phosphorylation. The effects of the pathogenic mutations Gly40Arg & Ala43Thr have been modelled in the context of the KHK structure. PMID: 19237742
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相關疾病：

Fructosuria (FRUCT)
蛋白家族：

Carbohydrate kinase PfkB family
組織特異性：

Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.
數據庫鏈接：

HGNC: 6315

OMIM: 229800

KEGG: hsa:3795

STRING: 9606.ENSP00000260598

UniGene: Hs.567297

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.