Human Intestinal-type alkaline phosphatase,ALPI ELISA Kit
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中文名稱(chēng)：人腸型堿性磷酸酶(ALPI)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E09709h
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人腸型堿性磷酸酶(ALPI)酶聯(lián)免疫試劑盒（CSB-E09709h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ALPI含量。ALPI即堿性磷酸酶腸同工酶。目前研究發(fā)現(xiàn)它與多種生理病理過(guò)程有關(guān)，如參與腸道內(nèi)物質(zhì)的代謝與吸收。其研究機(jī)制主要聚焦于它在腸道微環(huán)境中的作用，以及與腸道疾病、代謝性疾病發(fā)生發(fā)展的關(guān)聯(lián)，未來(lái)有望成為相關(guān)疾病診治新靶點(diǎn)。試劑盒檢測(cè)范圍為3.12 ng/mL-200 ng/mL，為腸道功能研究、疾病模型建立、營(yíng)養(yǎng)代謝機(jī)制探索等基礎(chǔ)科研領(lǐng)域提供可靠工具，尤其適用于評(píng)估腸道健康狀態(tài)、炎癥性腸病機(jī)制研究或藥物干預(yù)效果分析等實(shí)驗(yàn)場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名：Alkaline phosphatase intestinal ELISA Kit; Alkaline phosphomonoesterase ELISA Kit; Alpi ELISA Kit; Glycerophosphatase ELISA Kit; IAP ELISA Kit; Intestinal alkaline phosphatase ELISA Kit; Intestinal-type alkaline phosphatase ELISA Kit; Kasahara isozyme ELISA Kit; OTTHUMP00000164357 ELISA Kit; PPBI_HUMAN ELISA Kit
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縮寫(xiě)：ALP
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類(lèi)型：serum, plasma, tissue homogenates
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檢測(cè)范圍：3.12 ng/mL-200 ng/mL
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靈敏度：0.78 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Immunology
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human ALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 85 Range % 80-90 1:2 Average % 99 Range % 95-103 1:4 Average % 94 Range % 90-98 1:8 Average % 94 Range % 87-98 -
回收率：
The recovery of human ALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 89-96 EDTA plasma (n=4) 95 90-98 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 200 2.468 2.454 2.461 2.341 100 1.840 1.776 1.808 1.688 50 1.254 1.164 1.209 1.089 25 0.783 0.739 0.761 0.641 12.5 0.484 0.501 0.493 0.373 6.25 0.319 0.324 0.322 0.202 3.12 0.202 0.211 0.207 0.087 0 0.118 0.122 0.120 -
數(shù)據(jù)處理：
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貨期：3-5 working days
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Alkaline phosphatase that can hydrolyze various phosphate compounds.
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基因功能參考文獻(xiàn)：
- Data confirm that, in enterocytes (Caco-2 cells), 1-alpha,25-dihydroxy-vitamin-D3 up-regulates expression of 2 isoforms of IAP, alternative splicing variants. PMID: 28931466
- Expressions of human intestinal alkaline phosphatase and sucrase-isomaltase, which are intestinal differentiation markers, were highly enhanced in Caco-2 cells by menaquinone-4. PMID: 27865621
- Data indicate alkaline phosphatase (AP) as the primary soluble ectonucleotidase in infants undergoing cardiopulmonary bypass and show decreased capacity to clear AMP when AP activity decreases post-bypass. PMID: 27384524
- Intestinal alkaline phosphatase is a major regulator of gut mucosal permeability and appears to work at least partly through improving tight junction protein levels and localization. PMID: 27106638
- A High Level of Intestinal Alkaline Phosphatase Is Protective Against Type 2 Diabetes Mellitus Irrespective of Obesity PMID: 26844282
- Review of the role of intestinal alkaline phosphatase in inflammatory diseases. Loss of IAP expression or function is associated with increased intestinal inflammation, dysbiosis, bacterial translocation and subsequently systemic inflammation. PMID: 27083970
- The expression of ALPi and MUC5AC in cocultures of Caco-2 and HT29 cells developed for permeability studies is reported. PMID: 26299896
- Data indicate that histone deacetylase inhibitors (HDACi) induce intestinal alkaline phosphatase (ALPi) in a subset of colon cancer cell lines in a Kruppel-like factor 5 (KLF5)-dependent manner. PMID: 25037223
- Report lowered intestinal alkaline phosphatase in the colonic mucosa of children with inflammatory bowel disease. PMID: 22783049
- we are the first to demonstrate the alteration of protein expression of iAP in the duodenal mucosa of children with newly diagnosed coeliac disease PMID: 22262031
- Mechanism of IAP action appears to be through dephosphorylation of specific bacterial components, including LPS, CpG DNA, and flagellin, and not on live bacteria. IAP likely targets these bacterially derived molecules as gut mucosal defense factor. PMID: 20489044
- the structural differences in human AP isoforms are demonstrated through models PMID: 12372831
- intestinal alkaline transactivation by Kruppel-like factor-4 is likely mediated through a critical region located within the proximal IAP promoter region PMID: 12919939
- Northern blotting detected expression of two IAP transcripts, which were increased approximately 3-fold in response to thyroid hormone PMID: 15143152
- receptor for Aeromonas sobria hemolysin PMID: 15715171
- Cdx1 activates the IAP gene via a novel cis element, whereas Cdx2 inhibits the Cdx1 effects PMID: 15774940
- Cotransfection with an HNF-4alpha expression vector demonstrated a direct activation of the ALPI promoter through -94 to -82 element PMID: 15831710
- Crohn's disease increases the alkaline phosphatase activity in the intestine. Use histochemistry to differentiate Crohn's disease/ulcerative colitis. PMID: 17498884
- has the ability to detoxify lipopolysaccharide and prevent bacterial invasion across the gut mucosal barrier. IAP expression and function are lost with starvation and maintained by enteral feeding PMID: 18292227
- Cathepsin C propeptide interacts with intestinal alkaline phosphatase (IAP) and heat shock cognate protein 70. The propeptide of cathepsin C may stimulate the sorting to the lysosome contributing to the degradation of IAP in Caco-2 cells. PMID: 18307834
- Suggest role for lysophosphatidylcholine in brush-border intestinal alkaline phosphatase release and restoration. PMID: 19407215
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亞細(xì)胞定位：Cell membrane; Lipid-anchor, GPI-anchor.
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蛋白家族：Alkaline phosphatase family
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