Human Interferon alpha-21(IFNA21) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人干擾素α-21(IFNA21)酶聯免疫試劑盒（CSB-EL011039HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNA21含量。人干擾素α-21（IFNA21）是一種具有抗病毒和抗增殖活性的蛋白質，通過結合細胞表面受體IFNAR1和IFNAR2，激活JAK-STAT信號通路，調節免疫反應。其研究涉及基因改組技術，旨在提高其抗病毒和抗增殖活性，用于治療多種疾病。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，該產品適用于科研領域對感染性疾病、自身免疫反應或腫瘤微環境等研究中IFNA21表達水平的定量分析，可用于評估病毒感染模型、免疫調節實驗或藥物干預后生物樣本中IFNA21的動態變化，為探索其生物學功能及作用機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IFNA21 ELISA Kit; Interferon alpha-21 ELISA Kit; IFN-alpha-21 ELISA Kit; Interferon alpha-F ELISA Kit; LeIF F ELISA Kit
縮寫：

IFNA21
Uniprot No.：

P01568
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IFNA21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	91-101
1:2	Average %	104
	Range %	98-107
1:4	Average %	87
	Range %	83-91
1:8	Average %	94
	Range %	90-97

回收率：

The recovery of human IFNA21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	84-94
EDTA plasma (n=4)	101	96-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
亞細胞定位：

Secreted.
蛋白家族：

Alpha/beta interferon family
數據庫鏈接：

HGNC: 5424

OMIM: 147584

KEGG: hsa:3452

STRING: 9606.ENSP00000369574

UniGene: Hs.113211