Human Immunoglobulin J chain(IGJ) ELISA kit

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產品詳情

產品描述：

人免疫球蛋白j鏈(Ig-j)酶聯免疫試劑盒（CSB-E09237h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、唾液、尿液樣本中的IGJ含量。IGJ即免疫球蛋白J鏈，它參與分泌型免疫球蛋白的組裝與轉運。在研究機制上，其可調節免疫球蛋白多聚化，影響黏膜免疫功能。對IGJ的深入研究有助于理解免疫相關疾病發病機制，為開發針對免疫調節的治療方法提供理論支持。試劑盒檢測范圍為10 ng/mL-40000 ng/mL，適用于科研領域中對黏膜免疫機制、感染性疾病或免疫缺陷相關研究，也可用于評估母乳喂養中免疫成分傳遞效率、淚液或唾液局部免疫狀態分析等實驗場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IGCJ ELISA Kit; IGJ_HUMAN ELISA Kit; Immunoglobulin J chain ELISA Kit; Immunoglobulin J polypeptide linker protein for immunoglobulin alpha and mu polypeptides ELISA Kit; immunoglobulin joining chain ELISA Kit; JCH ELISA Kit; JCHAIN ELISA Kit; Joining chain of multimeric IgA and IgM ELISA Kit
縮寫：

IGJ
Uniprot No.：

P01591
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, saliva, urine, tear, breast milk
檢測范圍：

10 ng/mL-40000 ng/mL
靈敏度：

10 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IGJ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	89
	Range %	81-99
1:2000	Average %	95
	Range %	83-99
1:4000	Average %	95
	Range %	90-102
1:8000	Average %	90
	Range %	85-100

回收率：

The recovery of human IGJ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-99
EDTA plasma (n=4)	95	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces dimers and/or larger polymers. It also helps to bind these immunoglobulins to secretory component.
基因功能參考文獻：
1. TLR2 ligands induce CCR9 and CCR10 expression by circulating B-cells and increase their chemotaxis. TLR2 stimulation also induced J chain and IgA production demonstrating the induction of mucosal-like antibody secreting cells. PMID: 20947433
亞細胞定位：

Secreted.
數據庫鏈接：

HGNC: 5713

OMIM: 147790

KEGG: hsa:3512

STRING: 9606.ENSP00000254801

UniGene: Hs.643431