Human Ig gamma-3 chain C region(IGHG3) ELISA kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人Igγ3鏈C區(IGHG3)酶聯免疫試劑盒（CSB-EL011158HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的IGHG3含量。IGHG3（免疫球蛋白重鏈恒定區3）是一種免疫球蛋白的重鏈恒定區，參與免疫應答。研究顯示，IGHG3在肺結核病患者血清中的含量顯著高于健康對照組，可能與肺結核病的發病機制相關。其具體作用機制尚在研究中，但已有研究表明，IGHG3可能作為潛在生物標志物，有助于肺結核病的診斷。試劑盒檢測范圍為2.7 ng/mL-2000 ng/mL，該產品適用于科研領域中對IgG3亞型表達水平的動態監測，如探索自身免疫疾病病理機制、評估疫苗免疫效果、研究免疫調節藥物干預后的體液免疫變化等，為免疫學研究和生物標志物發現提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IGHG3Immunoglobulin heavy constant gamma 3 ELISA Kit; HDC ELISA Kit; Heavy chain disease protein ELISA Kit; Ig gamma-3 chain C region ELISA Kit
縮寫：

IGHG3
Uniprot No.：

P01860
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

2.7 ng/mL-2000 ng/mL
靈敏度：

2.7 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IGHG3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	99
	Range %	93-106
1:2000	Average %	90
	Range %	85-95
1:4000	Average %	87
	Range %	82-91
1:8000	Average %	93
	Range %	86-98

回收率：

The recovery of human IGHG3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-94
EDTA plasma (n=4)	95	90-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
亞細胞定位：

Secreted. Cell membrane.
數據庫鏈接：

HGNC: 5527

OMIM: 147120

UniGene: Hs.510635