Human Growth hormone variant(GH2) ELISA kit

Human Growth hormone variant(GH2) ELISA kit

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中文名稱：

人生長激素變體(GH2)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL009408HU
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人生長激素變體(GH2)酶聯(lián)免疫試劑盒（CSB-EL009408HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的GH2含量。試劑盒檢測(cè)范圍為133 pg/mL-4000 pg/mL，支持科研人員在體外實(shí)驗(yàn)中探究GH2在生長發(fā)育調(diào)控、內(nèi)分泌信號(hào)通路或疾病模型中的分子機(jī)制，為相關(guān)領(lǐng)域提供可靠的定量分析工具；適用于生命科學(xué)領(lǐng)域的基礎(chǔ)研究本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

GH2 ELISA Kit; Growth hormone variant ELISA Kit; GH-V ELISA Kit; Growth hormone 2 ELISA Kit; Placenta-specific growth hormone ELISA Kit
縮寫：

GH2
Uniprot No.：

P01242
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

133 pg/mL-4000 pg/mL
靈敏度：

33 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human GH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	83-98
1:2	Average %	90
	Range %	85-97
1:4	Average %	94
	Range %	90-101
1:8	Average %	94
	Range %	86-102

回收率：

The recovery of human GH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	93	87-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.531	2.447	2.489	2.443
1666.7	1.295	1.342	1.319	1.273
666.7	0.628	0.607	0.618	0.572
266.7	0.285	0.306	0.296	0.250
133	0.163	0.152	0.158	0.112
0	0.045	0.047	0.046

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
基因功能參考文獻(xiàn)：
1. Type 1 diabetic women delivering large for gestational age infants had lower placental GH levels in early pregnancy. PMID: 26589570
2. genetic association studies in populations in Estonia, Czech Republic, and Bashkortostan/Russia: Data suggest an SNP in GH2 (rs2006123) is associated with adult height (not body mass index); A-allele is associated with taller stature. [META-ANALYSIS] PMID: 24035309
3. Maternal serum PGH at 11-13 weeks' gestation is unlikely to be a useful biochemical marker for early prediction of small for gestational age neonates. PMID: 22489624
4. results are consistent with a pleiotropic effect of placental hGH/CSH genes at the maternal-fetal interface relating to the regulation of fetal growth and the risk of affected maternal metabolism. PMID: 22387044
5. These results suggest that the normal PGH-IGF-I-IGFBP3 axis in pregnancy is abnormal in Type 1 Diabetic pregnancies. PMID: 22363400
6. early first trimester maternal serum marker for Down syndrome PMID: 19844941
7. The hGH-V promoter activity and the endogenous mRNA expression in human placental choriocarcinoma cell line JAR in the presence of glucose and demethylating genome conditions, were compared. PMID: 20005926
8. GH-V plays a major role in affecting target cells expressing the GH receptor, thus potentially exerting significant GH-like effects on maternal physiology during pregnancy. PMID: 12213191
9. Data show that improved binding affinity of human growth hormone (hGH(v)) results from numerous small contributions distributed broadly over the interface. PMID: 12946357
10. Using a mutagenesis-scanning analysis of 81 single and 32 pairwise double mutations, it is shown that the GHv hormone's two spatially distal receptor binding sites (Site1 and Site2) are allosterically coupled PMID: 15563602
11. results offer the first evidence for a placental role of human placental growth hormone(hPGH) and suggest an autocrine/paracrine role of hPGH in the regulation of trophoblast invasion PMID: 15718272
12. PGH may play a role in the mechanisms of disease in preeclampsia and fetal growth restriction. PMID: 17701665
13. Placental growth hormone (PGH) secretion by BeWo cells is regulated by hormonal factors and adipokines. Elevated insulin and leptin levels might down-regulate PGH secretion. PMID: 18356738
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亞細(xì)胞定位：

Secreted.
蛋白家族：

Somatotropin/prolactin family
組織特異性：

Expressed in the placenta.
數(shù)據(jù)庫鏈接：

HGNC: 4262

OMIM: 139240

KEGG: hsa:2689

STRING: 9606.ENSP00000333157

UniGene: Hs.406754

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.