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中文名稱：人脂肪酸結合蛋白6(FABP6)酶聯免疫試劑盒
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貨號：CSB-EL007955HU
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規格：96T/48T
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價格：￥3600/￥2500
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促銷：
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其他：
產品詳情
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產品描述：人脂肪酸結合蛋白6(FABP6)酶聯免疫試劑盒（CSB-EL007955HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的FABP6含量。FABP6即脂肪酸結合蛋白6，在腸道膽汁酸轉運等過程中發揮作用。其研究機制主要聚焦在與膽汁酸代謝調控、脂代謝的關聯方面。探索其作用機制，有望為膽汁酸相關疾病、脂質代謝紊亂疾病等的治療提供新的靶點和思路。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，該產品適用于基礎科研中探索FABP6在腸道功能、代謝性疾病或癌癥發生發展中的作用機制，也可用于評估不同實驗條件下（如藥物處理、基因編輯）細胞或動物模型中FABP6的表達變化，為營養學、藥理學及病理學研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：Fabp6 ELISA Kit; FABP6_HUMAN ELISA Kit; Fatty acid binding protein 6; ileal (gastrotropin) ELISA Kit; Fatty acid-binding protein 6 ELISA Kit; Gastrotropin ELISA Kit; GT ELISA Kit; I 15P ELISA Kit; I BABP ELISA Kit; I BALB ELISA Kit; I BAP ELISA Kit; I-15P ELISA Kit; I-BABP ELISA Kit; I15P ELISA Kit; IBABP ELISA Kit; IBALB ELISA Kit; IBAP ELISA Kit; ILBP ELISA Kit; ILBP3 ELISA Kit; Ileal lipid binding protein ELISA Kit; Ileal lipid-binding protein ELISA Kit; ILLBP ELISA Kit; Intestinal 15 kDa protein ELISA Kit; Intestinal bile acid binding protein ELISA Kit; Intestinal bile acid-binding protein ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, tissue homogenates, cell lysates
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檢測范圍：0.312 ng/mL-20 ng/mL
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靈敏度：0.078 ng/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Signal Transduction
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human gastrotropin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 82-94 1:2 Average % 97 Range % 91-101 1:4 Average % 94 Range % 85-97 1:8 Average % 90 Range % 84-95 -
回收率：
The recovery of human gastrotropin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 100 91-104 EDTA plasma (n=4) 92 88-96 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 20 2.266 2.189 2.228 2.075 10 1.649 1.676 1.663 1.510 5 0.961 0.924 0.943 0.790 2.5 0.633 0.629 0.631 0.478 1.25 0.355 0.364 0.360 0.207 0.625 0.250 0.266 0.258 0.105 0.312 0.199 0.187 0.193 0.040 0 0.154 0.152 0.153 -
數據處理：
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貨期：3-5 working days
引用文獻
- Increased ileal bile acid binding protein and galectin-9 are associated with mild cognitive impairment and Alzheimer's disease Wang X, et al,Journal of Psychiatric Research,2019
產品評價
相關產品
靶點詳情
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功能：Binds to bile acids and is involved in enterohepatic bile acid metabolism. Required for efficient apical to basolateral transport of conjugated bile acids in ileal enterocytes. In vitro binds to bile acids in the order: deoxycholic acid > cholic acid > chenodeoxycholic acid and respective BA conjugation modifies affinities in the order taurine-conjugated > glycine-conjugated > unconjugated bile acids. Stimulates gastric acid and pepsinogen secretion.; Essential for the survival of colon cancer cells to bile acid-induced apoptosis.
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基因功能參考文獻：
- Structural determinants of ligand binding in the ternary complex of human ileal bile acid binding protein with glycocholate and glycochenodeoxycholate obtained from solution NMR PMID: 26613247
- Analysis of slow and fast motions in I-BABP indicates largely different energy landscapes for the apo and holo states suggesting that optimization of binding interactions might be achieved by altering the dynamic behavior of specific protein segments. PMID: 25073073
- show, using electrospray ionization mass spectroscopy, that human ILBP binds bile acids with a 3:1 ratio, even at low protein and ligand concentrations PMID: 23758264
- Ursodeoxycholic acid induces unique conformational changes in IBABP. PMID: 22223860
- NMR data are in agreement with a conformational selection model we proposed earlier for I-BABP and support the hypothesis of an allosteric mechanism of ligand binding PMID: 22329738
- The-putative functional-Thr79Met substitution of FABP6 confers a protective effect on type 2 diabetes in obese individuals. PMID: 19744871
- NMR structure of human ileal lipid-binding protein-cholyltaurine complex and its comparison with homologous structures PMID: 12486725
- the I-BABP gene may be a novel target for PPAR in humans PMID: 15936983
- ASBT and ILBP protein were 48% and 67% lower in normal weight gallstone carriers than in controls (P < 0.05); similar differences were found for mRNA expression levels. PMID: 16237211
- The expression of FABP6 was higher in primary colorectal cancers and adenomas than in normal epithelium, but was dramatically decreased in lymph node metastases, suggesting that FABP6 may play an important role in early carcinogenesis. PMID: 16951225
- In keeping with its role in the enterohepatic circulation and ileal reabsorption of bile acids, the gene promoter contains consensus elements for CDX2 and FXR. More than one transcription start site has been identified. PMID: 14654244
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亞細胞定位：[Isoform 1]: Cytoplasm. Membrane; Peripheral membrane protein; Cytoplasmic side.; [Isoform 2]: Cytoplasm.
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蛋白家族：Calycin superfamily, Fatty-acid binding protein (FABP) family
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組織特異性：Isoform 1 is expressed in the jejunum, ileum, cecum and ascending colon intestine. Isoform 2 is xpressed in the gallbladder, duodenum, jejunum, ileum, cecum, ascending, transverse and descending colon, sigmoid colon and rectum. Isoform 2 is expressed in c
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