Human Follicular dendritic cell secreted peptide(C4orf7) ELISA kit

說明書下載

中文名稱：

人濾泡樹突狀細胞分泌蛋白(C4orf7)酶聯(lián)免疫試劑盒
貨號：

CSB-EL003992HU
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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人濾泡樹突狀細胞分泌蛋白(C4orf7)酶聯(lián)免疫試劑盒（CSB-EL003992HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C4orf7含量。試劑盒檢測范圍為7.81 ng/mL-500 ng/mL，適用于免疫學基礎(chǔ)研究、疾病模型中的蛋白動態(tài)監(jiān)測以及相關(guān)分子通路的機制探索等科研場景，為深入解析該蛋白的生物學功能提供可靠技術(shù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

C4orf7 ELISA Kit; FDC secreted protein ELISA Kit; FDC-SP ELISA Kit; FDCSP ELISA Kit; FDSCP_HUMAN ELISA Kit; Follicular dendritic cell secreted peptide ELISA Kit; Follicular dendritic cell secreted protein ELISA Kit
縮寫：

C4orf7
Uniprot No.：

Q8NFU4
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.81 ng/mL-500 ng/mL
靈敏度：

1.95 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C4orf7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	105
	Range %	101-109
1:2	Average %	98
	Range %	94-103
1:4	Average %	87
	Range %	84-91
1:8	Average %	109
	Range %	105-114

回收率：

The recovery of human C4orf7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-93
EDTA plasma (n=4)	106	103-109

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
500	2.221	2.094	2.158	2.046
250	1.345	1.405	1.375	1.263
125	0.742	0.789	0.766	0.654
62.5	0.413	0.402	0.408	0.296
31.25	0.305	0.331	0.318	0.206
15.62	0.217	0.208	0.213	0.101
7.81	0.182	0.178	0.180	0.068
0	0.111	0.113	0.112

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Can bind to the surface of B-lymphoma cells, but not T-lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.
基因功能參考文獻：
1. These studies show that TNF-alpha stimulates human FDC-SP gene transcription by targeting YY1, GATA, C/EBP2 and C/EBP3 in the FDC-SP gene promoter. PMID: 29356241
2. LPS-induced upregulation of FDC-SP expression in human periodontal ligament cells may enhance osteoclastogenesis in periodontal disease. PMID: 26577469
3. FDC-SP may be involved in IgA production in the tonsils of Immunoglobulin A nephropathy patients. PMID: 25953661
4. FDC-SP overexpression inhibits osteogenic differentiation of hPDLCs. study contributes to a better understanding of biological functions governing FDC-SP-induced hPDLC differentiation. PMID: 24138099
5. Our findings demonstrate that transfection with FDC-SP has negligible adverse effect on proliferation of hPDLCs and imply the biological function of FDC-SP as a fibroblastic phenotype stabilizer PMID: 24357406
6. The role of C4orf7 in ovarian cancer cell morphology, motility and invasion was demonstrated. PMID: 20811673
7. FDC-SP has a very restricted tissue distribution, expressed by activated FDCs from tonsils and TNF-alpha-activated FDC-like cell lines, but not by B cell lines, primary germinal center B cells, or anti-CD40 plus IL-4-activated B cells. PMID: 12193705
8. Since only normal tissue was examined, these findings suggest that FDC-SP plays an important but previously unsuspected role within oral connective tissue. PMID: 16259954
9. These results provide the first evidence for immunomodulatory activities of FDC-SP and implicate this molecule as a regulator of B cell responses. PMID: 17548624
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亞細胞定位：

Secreted.
組織特異性：

Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon.
數(shù)據(jù)庫鏈接：

HGNC: 19215

OMIM: 607241

KEGG: hsa:260436

STRING: 9606.ENSP00000318437

UniGene: Hs.733448

Human Follicular dendritic cell secreted peptide(C4orf7) ELISA kit

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