Human F-actin ELISA Kit

產(chǎn)品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人F-肌動蛋白酶聯(lián)免疫試劑盒（CSB-E13678h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的F-actin含量。F-actin即絲狀肌動蛋白，是細胞骨架的重要組成部分。其在細胞形態(tài)維持、運動遷移、物質(zhì)運輸?shù)冗^程中發(fā)揮關(guān)鍵作用。研究機制上，主要聚焦于其聚合和解聚動態(tài)平衡調(diào)控，以及與多種結(jié)合蛋白相互作用，影響細胞生理和病理狀態(tài)。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于細胞骨架動態(tài)研究、細胞力學特性分析、心血管疾病或腫瘤轉(zhuǎn)移機制探索等科研領(lǐng)域，尤其可為細胞應激反應、病理模型構(gòu)建及藥物干預效果評估提供可靠的分子檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

F-actin
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human F-actin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of human F-actin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-101
EDTA plasma (n=4)	88	85-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.