Human Epigen(EPGN) ELISA kit

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產品詳情

產品描述：

人Epigen(EPGN)酶聯免疫試劑盒（CSB-EL007719HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EPGN含量。EPGN 即表皮生長因子樣蛋白，在多種腫瘤中異常表達。其作為細胞膜生長因子，可激活下游信號通路促進細胞增殖、遷移等。目前圍繞 EPGN 的研究多聚焦于其在腫瘤發生發展中的機制，有望為腫瘤診斷和治療提供新靶點和思路。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，本試劑盒適用于體外研究EPGN在腫瘤生物學、細胞信號通路或炎癥相關分子機制中的表達特征，支持多種樣本類型檢測，具有高靈敏度和特異性，可滿足基礎科研中對EPGN蛋白表達動態變化的精確分析需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

ALGV3072 ELISA Kit; EPG ELISA Kit; EPGN ELISA Kit; EPGN_HUMAN ELISA Kit; Epigen ELISA Kit; Epigen precursor ELISA Kit; Epithelial mitogen ELISA Kit; PRO9904 ELISA Kit
縮寫：

EPGN
Uniprot No.：

Q6UW88
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human EPGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	96-107
1:2	Average %	102
	Range %	97-105
1:4	Average %	92
	Range %	85-96
1:8	Average %	97
	Range %	92-101

回收率：

The recovery of human EPGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	105	98-109

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.
基因功能參考文獻：
1. Study shows how the EGFR ligands epiregulin (EREG) and epigen (EPGN) stabilize different dimeric conformations of the EGFR extracellular region. Results reveal how responses to different EGFR ligands are defined by receptor dimerization strength and signaling dynamics. These findings have broad implications for understanding receptor tyrosine kinase (RTK) signaling specificity. PMID: 28988771
2. cloning of a related gene in mouse PMID: 112783
3. EPG is an EGF-like growth factor and a low affinity ligand PMID: 15611079
亞細胞定位：

[Isoform 1]: Membrane; Single-pass type I membrane protein.; [Isoform 2]: Membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted.; [Isoform 4]: Secreted.; [Isoform 5]: Secreted.; [Isoform 6]: Secreted.
數據庫鏈接：

HGNC: 17470

KEGG: hsa:255324

UniGene: Hs.401237