Human Epigen(EPGN) ELISA kit

文獻(xiàn)引用 (1)

中文名稱：

人Epigen(EPGN)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL007719HU
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
引用文獻(xiàn)
相關(guān)產(chǎn)品
相關(guān)問答
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

人Epigen(EPGN)酶聯(lián)免疫試劑盒（CSB-EL007719HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的EPGN含量。EPGN 即表皮生長(zhǎng)因子樣蛋白，在多種腫瘤中異常表達(dá)。其作為細(xì)胞膜生長(zhǎng)因子，可激活下游信號(hào)通路促進(jìn)細(xì)胞增殖、遷移等。目前圍繞 EPGN 的研究多聚焦于其在腫瘤發(fā)生發(fā)展中的機(jī)制，有望為腫瘤診斷和治療提供新靶點(diǎn)和思路。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL，本試劑盒適用于體外研究EPGN在腫瘤生物學(xué)、細(xì)胞信號(hào)通路或炎癥相關(guān)分子機(jī)制中的表達(dá)特征，支持多種樣本類型檢測(cè)，具有高靈敏度和特異性，可滿足基礎(chǔ)科研中對(duì)EPGN蛋白表達(dá)動(dòng)態(tài)變化的精確分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

ALGV3072 ELISA Kit; EPG ELISA Kit; EPGN ELISA Kit; EPGN_HUMAN ELISA Kit; Epigen ELISA Kit; Epigen precursor ELISA Kit; Epithelial mitogen ELISA Kit; PRO9904 ELISA Kit
縮寫：

EPGN
Uniprot No.：

Q6UW88
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human EPGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	96-107
1:2	Average %	102
	Range %	97-105
1:4	Average %	92
	Range %	85-96
1:8	Average %	97
	Range %	92-101

回收率：

The recovery of human EPGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	105	98-109

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.687	2.568	2.628	2.528
50	2.143	2.002	2.073	1.973
25	1.298	1.277	1.288	1.188
12.5	0.732	0.705	0.719	0.619
6.25	0.414	0.393	0.404	0.304
3.12	0.263	0.252	0.258	0.158
1.56	0.172	0.168	0.170	0.070
0	0.101	0.099	0.100

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

引用文獻(xiàn)

Candidalysin activates innate epithelial immune responses via epidermal growth factor receptor Ho J,Nature Communications,2019

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.
基因功能參考文獻(xiàn)：
1. Study shows how the EGFR ligands epiregulin (EREG) and epigen (EPGN) stabilize different dimeric conformations of the EGFR extracellular region. Results reveal how responses to different EGFR ligands are defined by receptor dimerization strength and signaling dynamics. These findings have broad implications for understanding receptor tyrosine kinase (RTK) signaling specificity. PMID: 28988771
2. cloning of a related gene in mouse PMID: 112783
3. EPG is an EGF-like growth factor and a low affinity ligand PMID: 15611079
亞細(xì)胞定位：

[Isoform 1]: Membrane; Single-pass type I membrane protein.; [Isoform 2]: Membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted.; [Isoform 4]: Secreted.; [Isoform 5]: Secreted.; [Isoform 6]: Secreted.
數(shù)據(jù)庫鏈接：

HGNC: 17470

KEGG: hsa:255324

UniGene: Hs.401237

Human Epigen(EPGN) ELISA kit

產(chǎn)品詳情

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

相關(guān)產(chǎn)品

相關(guān)問答

靶點(diǎn)詳情

Most popular with customers

產(chǎn)品中心

信號(hào)通路

技術(shù)信息

聯(lián)系信息