Human Epidermal growth factor-like protein 6(EGFL6) ELISA kit

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中文名稱：

人表皮生長因子樣蛋白6(EGFL6)酶聯免疫試劑盒
貨號：

CSB-EL007475HU
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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人表皮生長因子樣蛋白6(EGFL6)酶聯免疫試劑盒（CSB-EL007475HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EGFL6含量。EGFL6作為靶點，其它在人體生理或病理過程中具有一定作用。研究機制上，可能涉及參與細胞間信號傳導、影響細胞的增殖、遷移等活動，目前科學家正深入研究其在疾病發生發展中的具體作用機制，以探尋相關治療策略。試劑盒檢測范圍為78 pg/mL-5000 pg/mL，適用于體外研究EGFL6在腫瘤發生發展、血管生成相關疾病中的表達模式，也可用于細胞培養上清或組織樣本的蛋白動態監測，為探索其分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

EGF like domain multiple 6 ELISA Kit; EGF repeat containing protein 6 ELISA Kit; EGF-like protein 6 ELISA Kit; EGF-like-domain multiple 6 ELISA Kit; egfl6 ELISA Kit; EGFL6_HUMAN ELISA Kit; epidermal growth factor like protein 6 ELISA Kit; Epidermal growth factor-like protein 6 ELISA Kit; MAEG ELISA Kit; MAM and EGF domain containing ELISA Kit; MAM and EGF domains containing gene protein ELISA Kit; MAM and EGF domains-containing gene protein ELISA Kit; W80 ELISA Kit
縮寫：

EGFL6
Uniprot No.：

Q8IUX8
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

78 pg/mL-5000 pg/mL
靈敏度：

19.5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human EGFL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	97-108
1:2	Average %	101
	Range %	93-105
1:4	Average %	97
	Range %	89-101
1:8	Average %	104
	Range %	94-109

回收率：

The recovery of human EGFL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-94
EDTA plasma (n=4)	102	95-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	1.972	1.918	1.945	1.800
2500	1.400	1.439	1.420	1.275
1250	1.072	1.025	1.049	0.904
625	0.762	0.734	0.748	0.603
312	0.521	0.529	0.525	0.380
156	0.348	0.357	0.353	0.208
78	0.271	0.283	0.277	0.132
0	0.148	0.142	0.145

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Elevated EGFL6 modulates cell metastasis and growth via AKT pathway in nasopharyngeal carcinoma Zhu Z, et al,Cancer Medicine,2018
Molecular biomarkers for weight control in obese individuals subjected to a multi-phase dietary intervention Bolton J.et al,J Clin Endocrinol Metab.,2017

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功能：

May bind integrin alpha-8/beta-1 and play a role in hair follicle morphogenesis. Promotes matrix assembly.
基因功能參考文獻：
1. In conclusion, our results suggest that plasma level of EGFL6 may be useful to assess disease progression, and especially advanced T status and higher TNM stage in patients with oral squamous cell carcinoma. PMID: 28539817
2. EGFL6 is an angiocrine and tumor cell factor that promotes tumorigenesis via SHP2-mediated signaling. EGFL6 stimulates ALDH+ ovarian cancer stem cell to migrate and undergo asymmetric division. PMID: 27803106
3. EGFL6 was overexpressed in benign meningioma tissues and serum. PMID: 23285163
4. results indicate that human EGFL6 is a paracrine/autocrine growth factor of adipose tissue up-regulated in obesity and potentially involved in the process of adipose tissue expansion and the development of obesity PMID: 20574786
5. There is an absence of GNAS and EGFL6 mutations in common human cancers. PMID: 18038328
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亞細胞定位：

Secreted, extracellular space, extracellular matrix, basement membrane.
蛋白家族：

Nephronectin family
數據庫鏈接：

HGNC: 3235

OMIM: 300239

KEGG: hsa:25975

STRING: 9606.ENSP00000369976

UniGene: Hs.12844

Human Epidermal growth factor-like protein 6(EGFL6) ELISA kit

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