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Human EGF, latrophilin and seven transmembrane domain-containing protein 1(ELTD1) ELISA kit

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產品詳情

產品描述：

人EGF、嗜乳蛋白和7跨膜結構域蛋白1(ELTD1)酶聯免疫試劑盒（CSB-EL007630HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的ELTD1含量。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，適用于基礎醫學研究中相關蛋白的表達水平檢測，例如細胞功能調控機制探索、疾病動物模型分析或生物標志物篩選等實驗場景，為腫瘤生物學、再生醫學及代謝疾病研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

ADGRL4; ELTD1; ETL; UNQ202/PRO228; Adhesion G protein-coupled receptor L4; EGF, latrophilin and seven transmembrane domain-containing protein 1; EGF-TM7-latrophilin-related protein; ETL protein
縮寫：

ELTD1
Uniprot No.：

Q9HBW9
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

25 pg/mL-1600 pg/mL
靈敏度：

6.25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ELTD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-98
1:2	Average %	97
	Range %	91-103
1:4	Average %	92
	Range %	87-98
1:8	Average %	88
	Range %	85-92

回收率：

The recovery of human ELTD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	98-104
EDTA plasma (n=4)	87	82-91

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Endothelial orphan receptor that acts as a key regulator of angiogenesis.
基因功能參考文獻：
1. High ELTD1 expression is associated with glioma. PMID: 27416955
2. miR-139-5p suppresses glioblastoma multiforme cell proliferation by targeting ELTD1 and regulating cell cycle. PMID: 26449464
3. Results provide insight into the regulation of tumor angiogenesis and highlight ELTD1 as key player in blood vessel formation. PMID: 23871637
4. Two of these (D1S0818i-ELTD1 and D6S0035i-MAPK14) remain associated following application of Bonferroni's correction and multivariate analysis. PMID: 23474535
5. Results of this study strongly suggests that associative analysis was able to accurately identify ELTD1 as a putative glioma-associated biomarker PMID: 23096411
6. Genes such as ELTD1 on chromosome 1, in addition to genes on chromosomes 4 (eg, GABRA2) and 6 (eg, CNR1), may be associated with the genetic risk for cannabis use disorders. PMID: 18519829
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亞細胞定位：

Cell membrane; Multi-pass membrane protein.
蛋白家族：

G-protein coupled receptor 2 family, Adhesion G-protein coupled receptor (ADGR) subfamily
組織特異性：

Detected in the majority of epithelial cells in tumor and normal tissues. Expressed also in human umbilical vein endothelial cells.
數據庫鏈接：

HGNC: 20822

OMIM: 616419

KEGG: hsa:64123

STRING: 9606.ENSP00000359778

UniGene: Hs.132314