Human Desert hedgehog (DHH) ELISA kit
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中文名稱：人沙漠刺猬因子(DHH)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E12006h
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人沙漠刺猬因子(DHH)酶聯(lián)免疫試劑盒（CSB-E12006h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的DHH含量。靶點(diǎn)研究是藥物研發(fā)的關(guān)鍵，旨在識(shí)別與疾病相關(guān)的生物分子，如蛋白質(zhì)、酶或信號(hào)通路。通過(guò)理解靶點(diǎn)的作用機(jī)制，研究人員可設(shè)計(jì)針對(duì)這些靶點(diǎn)的藥物，以治療疾病。靶點(diǎn)研究涉及基因突變分析、信號(hào)通路解析和藥物篩選等多個(gè)環(huán)節(jié)。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，支持科研領(lǐng)域?qū)HH蛋白表達(dá)水平的精準(zhǔn)分析，例如基礎(chǔ)研究中信號(hào)通路機(jī)制探索、疾病模型中DHH動(dòng)態(tài)變化的系統(tǒng)性研究，或在藥物開(kāi)發(fā)過(guò)程中評(píng)估干預(yù)措施對(duì)Hedgehog通路的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
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別名：C78960 ELISA Kit; Desert hedgehog (Drosophila) homolog ELISA Kit; Desert hedgehog ELISA Kit; Desert hedgehog homolog (Drosophila) ELISA Kit; Desert hedgehog protein C-product ELISA Kit; Desert hedgehog protein precursor ELISA Kit; DHH ELISA Kit; DHH_HUMAN ELISA Kit; GDXYM ELISA Kit; HHG-3 ELISA Kit; Hira ELISA Kit; SRXY7 ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, cell culture supernates, tissue homogenates
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檢測(cè)范圍：15.6 pg/mL-1000 pg/mL
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靈敏度：3.9 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Stem Cells
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human DHH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:5 Average % 89 Range % 84-92 1:10 Average % 99 Range % 95-101 1:20 Average % 99 Range % 95-102 1:40 Average % 95 Range % 90-98 -
回收率：
The recovery of human DHH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 91-96 EDTA plasma (n=4) 96 94-98 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 1000 2.418 2.402 2.410 2.297 500 1.766 1.880 1.823 1.710 250 1.223 1.213 1.218 1.105 125 0.689 0.707 0.698 0.585 62.5 0.453 0.432 0.443 0.330 31.2 0.324 0.331 0.328 0.215 15.6 0.224 0.218 0.221 0.108 0 0.115 0.111 0.113 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.
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基因功能參考文獻(xiàn)：
- Whole-exome sequencing revealed a homozygous variant in DHH leading to the p.Trp173Cys substitution. The relevant Trp residue is conserved, and its alteration was predicted to be deleterious. Molecular dynamics simulations showed that the mutation increases the conformational flexibility of the protein PMID: 28708305
- Single nucleotide polymorphism in the DHH gene is associated with bipolar disorder. PMID: 25517604
- Mutations in DHH play a role in 46,XY gonadal dysgenesis and are associated with seminoma formation and a neuropathy with minifascicle formation. PMID: 25927242
- Findings are unprecedented and indicate that the DHH-RHEBL1 fusion transcript is a novel recurrent feature in the changing landscape of CBFA2T3-GLIS2-positive childhood AML. PMID: 24127550
- Mutations in DHH are associated with 46,XY pure gonadal dysgenesis and mixed gonadal dysgenesis. PMID: 23786321
- This study demonistrated that Desert hedgehog links transcription factor Sox10 to peripheral nerve development. PMID: 22514309
- Studies indicate that pathways of Hedgehog (Hh), Wnt and Notch, which regulate development during embryonic life and somatic stem cells (SCs) in the adult organism, can be reactivated in malignancies and support tumor-initiating cells (TIC) scompartment. PMID: 22123293
- Two cases have been described in Indian patients with 46,XY complete gonadal dysgenesis that could be attributable to mutations in the Desert hedgehog (DHH) gene leading to non-functional DHH protein. PMID: 21816240
- We found no significant correlation between the expression of SOX9 and desert hedgehog, and neither SOX9 nor desert hedgehog expression was correlated to the histoprognostic grade in sarcomas. PMID: 21193222
- These data demonstrate that DHH is a key molecule in both male gonadal differentiation and perineurial formation in peripheral nerves PMID: 11990454
- Importance of DHH in mammalian male sexual differentiation, providing extended evidence that DHH constitutes a key gene in gonadal differentiation PMID: 16390857
- its signal transduction regulates tumor development. (review) PMID: 18788453
- Hh signaling is important in the pathogenesis of B-CLL and, hence, may be a potential therapeutic target PMID: 19074837
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相關(guān)疾病：Partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD); 46,XY sex reversal 7 (SRXY7)
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亞細(xì)胞定位：[Desert hedgehog protein N-product]: Cell membrane; Lipid-anchor; Extracellular side.; [Desert hedgehog protein C-product]: Secreted, extracellular space.
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蛋白家族：Hedgehog family
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數(shù)據(jù)庫(kù)鏈接：
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