Human Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen, CTX-I ELISA Kit

Human Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen, CTX-I ELISA Kit

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中文名稱：

人Ⅰ型膠原交聯(lián)羧基末端肽(CTX-I)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E11224h
規(guī)格：

96T/48T
價(jià)格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人Ⅰ型膠原交聯(lián)羧基末端肽(CTX-I)酶聯(lián)免疫試劑盒（CSB-E11224h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CTX-I含量。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL，常用于骨質(zhì)疏松機(jī)制研究、骨關(guān)節(jié)疾病模型評(píng)估及抗骨吸收藥物開(kāi)發(fā)等科研領(lǐng)域；適用于體外骨代謝標(biāo)志物檢測(cè)、細(xì)胞模型骨吸收活性分析及動(dòng)物實(shí)驗(yàn)樣本評(píng)估等非臨床研究場(chǎng)景，為骨生物學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

Cross-linked Carboxy-terminal telopeptide of type 1 collagen, CTX-1, Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I
縮寫(xiě)：

CTX-I
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CTX-I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-95
1:2	Average %	95
	Range %	91-98
1:4	Average %	97
	Range %	90-103
1:8	Average %	96
	Range %	91-101

回收率：

The recovery of human CTX-I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-92
EDTA plasma (n=4)	96	93-106

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.995	2.960	2.978	2.838
20	2.557	2.570	2.564	2.424
10	1.666	1.765	1.716	1.576
5	0.839	0.886	0.863	0.723
2.5	0.491	0.524	0.508	0.368
1.25	0.263	0.251	0.257	0.117
0.625	0.201	0.212	0.207	0.067
0	0.138	0.142	0.140

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.