Human Complement fragment 4b,C4b ELISA Kit

Human Complement fragment 4b,C4b ELISA Kit

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中文名稱：

人補(bǔ)體片斷4b(C4b)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E09302h
規(guī)格：

96T/48T
價(jià)格：

￥3200/￥2500
促銷：
其他：

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產(chǎn)品描述：

人補(bǔ)體片斷4b(C4b)酶聯(lián)免疫試劑盒（CSB-E09302h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的C4b含量。C4b是補(bǔ)體系統(tǒng)經(jīng)典途徑和凝集素途徑激活過程產(chǎn)生的重要片段。補(bǔ)體系統(tǒng)在免疫防御等方面發(fā)揮關(guān)鍵作用。研究顯示，C4b可參與調(diào)理吞噬、免疫黏附等過程，其異常活動(dòng)可能與多種免疫相關(guān)疾病發(fā)生發(fā)展有關(guān)，是免疫學(xué)研究重要靶點(diǎn)。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，適用于科研領(lǐng)域中對(duì)補(bǔ)體系統(tǒng)功能的探索，例如在炎癥模型、免疫調(diào)節(jié)實(shí)驗(yàn)或疾病機(jī)制研究中定量分析血清/血漿樣本的C4b表達(dá)動(dòng)態(tài)，為揭示補(bǔ)體介導(dǎo)的生理病理過程提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Basic complement C4 ELISA Kit; C2B5 ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 ELISA Kit; C4b ELISA Kit; C4B1 ELISA Kit; C4B2 ELISA Kit; C4B3 ELISA Kit; CO4B_HUMAN ELISA Kit; Complement C4 gamma chain ELISA Kit; Complement component 4B ELISA Kit; CPAMD3 ELISA Kit
縮寫：

C4b
Uniprot No.：

P0C0L5
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

15.6 ng/mL-1000 ng/mL
靈敏度：

3.9 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C4b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	84
	Range %	80-94
1:400	Average %	97
	Range %	91-102
1:800	Average %	98
	Range %	92-105
1:1600	Average %	92
	Range %	86-99

回收率：

The recovery of human C4b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-98
EDTA plasma (n=4)	98	90-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.566	2.366	2.466	2.373
500	2.031	1.931	1.981	1.888
250	1.409	1.319	1.364	1.271
125	0.867	0.854	0.861	0.768
62.5	0.524	0.514	0.519	0.426
31.2	0.345	0.325	0.335	0.242
15.6	0.213	0.203	0.208	0.115
0	0.094	0.091	0.093

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Intraperitoneal MASP-1 Levels are Associated with Peritoneal Solute Transport Rate in Peritoneal Dialysis Patients: A Retrospective Cohort Study J Li, Y Xiong, M Chen, D Xu, L Zhou,Journal of Inflammation Research,2024
Elevated complement mediator levels in endothelial-derived plasma exosomes implicate endothelial innate inflammation in diminished brain function of aging humans FM Elahi,Scientific Reports,2021
Traumatic brain injury increases plasma astrocyte┸\derived exosome levels of neurotoxic complement proteins Goetzl EJ,FASEB,2020
High complement levels in astrocyte-derived exosomes of Alzheimer\'s disease Goetzl EJ.et al,Ann Neurol,2018
Serum complement C4b, fibronectin, and prolidase are associated with the pathological changes of pulmonary tuberculosis Wang C et al,BMC Infect Dis,2014

功能：

Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.; Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
相關(guān)疾病：

Systemic lupus erythematosus (SLE); Complement component 4B deficiency (C4BD)
亞細(xì)胞定位：

Secreted. Cell junction, synapse. Cell projection, axon. Cell projection, dendrite.
組織特異性：

Complement component C4 is expressed at highest levels in the liver, at moderate levels in the adrenal cortex, adrenal medulla, thyroid gland, and the kidney, and at lowest levels in the heart, ovary, small intestine, thymus, pancreas and spleen. The extr
數(shù)據(jù)庫鏈接：

HGNC: 1324

OMIM: 120820

KEGG: hsa:100293534

STRING: 9606.ENSP00000415941

UniGene: Hs.534847

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.