Human Complement fragment 3b,C3b ELISA Kit

文獻引用 (5) 評價 (1) 說明書下載

中文名稱：

人補體片斷3b(C3b)酶聯免疫試劑盒
貨號：

CSB-E09303h
規格：

96T/48T
價格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產品詳情
產品評價
引用文獻
相關產品

產品詳情

產品描述：

人補體片斷3b(C3b)酶聯免疫試劑盒（CSB-E09303h）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、腦脊液（CSF）、唾液樣本中的C3b含量。C3b是補體系統中的重要分子。其背景在于補體系統是免疫防御的關鍵部分。研究機制方面，C3b參與調理吞噬作用，能與病原體結合促進吞噬細胞識別吞噬；還參與補體激活的旁路途徑，在免疫調控、炎癥反應等過程中發揮著重要作用。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，本產品適用于基礎醫學研究中對補體系統活性的動態監測，例如探究感染性疾病、神經炎癥或慢性炎癥模型中C3b的生物學功能，也可用于評估藥物或生物制劑對補體活化途徑的調控作用。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

C3b
種屬：

Homo sapiens (Human)
樣本類型：

serum, urine, cerebrospinal fluid (CSF), saliva
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C3b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

82-92

1:2

Average %

Range %

92-103

1:4

Average %

Range %

93-103

1:8

Average %

Range %

89-98

回收率：

The recovery of human C3b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-98
EDTA plasma (n=4)	97	91-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.115	2.003	2.059	1.932
100	1.748	1.736	1.742	1.615
50	1.245	1.266	1.256	1.129
25	0.756	0.782	0.769	0.642
12.5	0.568	0.572	0.570	0.443
6.25	0.333	0.342	0.338	0.211
3.12	0.199	0.187	0.193	0.066
0	0.128	0.126	0.127

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

The activation of complement C5a-C5aR1 axis in astrocytes facilitates the neuropathogenesis due to EV-A71 infection by upregulating CXCL1 P Zhu, W Ji, D Li, F Wang, T Sun, H Yang,Journal of Virology,2024
Predictive value of soluble CD59 for poor 28-day neurological prognosis and all-cause mortality in patients after cardiopulmonary resuscitation: a prospective observatory study L Wang,Journal of intensive care,2023
Intrathecal complement activation by the classical pathway in tick-borne encephalitis Veje M, et al,Journal of neurovirology,2019
Diminished expression of β2-GPI is associated with a reduced ability to mitigate complement activation in anti-GPIIb/IIIa-mediated immune thrombocytopenia Zhu X.et al,Ann Hematol,2017
Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis. Eriksson CE.et al,J Neuroimmunol.,2016

產品評價

平均分:

5.0分 - 1 個評價

樣品類型：生物流體肺泡灌洗液

樣品信息：人

稀釋比：其他 1：2

產品評價：我使用CSB-E09303h檢測MPP組以及無肺炎表現異物堵塞患者肺泡灌洗液中C3b濃度，空白稀釋液OD為0.163，標準曲線的R=0.999258，樣本稀釋2倍后進行檢測，極個別（3個）樣本濃度低于檢測下限，但考慮到部分樣本濃度偏高，仍建議使用兩倍稀釋進行實驗。試劑盒符合實驗要求，推薦使用

By Z老師

Human Complement fragment 3b,C3b ELISA Kit

產品詳情

引用文獻

產品評價

相關產品

Most popular with customers

產品中心

信號通路

技術信息

聯系信息