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中文名稱：人補體片斷3b(C3b)酶聯(lián)免疫試劑盒
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貨號：CSB-E09303h
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規(guī)格：96T/48T
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價格：￥3200/￥2500
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促銷：
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人補體片斷3b(C3b)酶聯(lián)免疫試劑盒（CSB-E09303h）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、腦脊液（CSF）、唾液樣本中的C3b含量。C3b是補體系統(tǒng)中的重要分子。其背景在于補體系統(tǒng)是免疫防御的關(guān)鍵部分。研究機制方面，C3b參與調(diào)理吞噬作用，能與病原體結(jié)合促進吞噬細胞識別吞噬；還參與補體激活的旁路途徑，在免疫調(diào)控、炎癥反應(yīng)等過程中發(fā)揮著重要作用。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，本產(chǎn)品適用于基礎(chǔ)醫(yī)學(xué)研究中對補體系統(tǒng)活性的動態(tài)監(jiān)測，例如探究感染性疾病、神經(jīng)炎癥或慢性炎癥模型中C3b的生物學(xué)功能，也可用于評估藥物或生物制劑對補體活化途徑的調(diào)控作用。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫：C3b
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種屬：Homo sapiens (Human)
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樣本類型：serum, urine, cerebrospinal fluid (CSF), saliva
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檢測范圍：3.12 ng/mL-200 ng/mL
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靈敏度：0.78 ng/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Immunology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human C3b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 87 Range % 82-92 1:2 Average % 98 Range % 92-103 1:4 Average % 99 Range % 93-103 1:8 Average % 94 Range % 89-98 -
回收率：
The recovery of human C3b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 90-98 EDTA plasma (n=4) 97 91-99 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 200 2.115 2.003 2.059 1.932 100 1.748 1.736 1.742 1.615 50 1.245 1.266 1.256 1.129 25 0.756 0.782 0.769 0.642 12.5 0.568 0.572 0.570 0.443 6.25 0.333 0.342 0.338 0.211 3.12 0.199 0.187 0.193 0.066 0 0.128 0.126 0.127 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻
- The activation of complement C5a-C5aR1 axis in astrocytes facilitates the neuropathogenesis due to EV-A71 infection by upregulating CXCL1 P Zhu, W Ji, D Li, F Wang, T Sun, H Yang,Journal of Virology,2024
- Predictive value of soluble CD59 for poor 28-day neurological prognosis and all-cause mortality in patients after cardiopulmonary resuscitation: a prospective observatory study L Wang,Journal of intensive care,2023
- Intrathecal complement activation by the classical pathway in tick-borne encephalitis Veje M, et al,Journal of neurovirology,2019
- Diminished expression of β2-GPI is associated with a reduced ability to mitigate complement activation in anti-GPIIb/IIIa-mediated immune thrombocytopenia Zhu X.et al,Ann Hematol,2017
- Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis. Eriksson CE.et al,J Neuroimmunol.,2016
產(chǎn)品評價
平均分:
樣品類型：生物流體 肺泡灌洗液
樣品信息：人
稀釋比：其他 1：2
產(chǎn)品評價： 我使用CSB-E09303h檢測MPP組以及無肺炎表現(xiàn)異物堵塞患者肺泡灌洗液中C3b濃度，空白稀釋液OD為0.163，標(biāo)準(zhǔn)曲線的R=0.999258，樣本稀釋2倍后進行檢測，極個別（3個）樣本濃度低于檢測下限，但考慮到部分樣本濃度偏高，仍建議使用兩倍稀釋進行實驗。試劑盒符合實驗要求，推薦使用
By Z老師
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