Human Complement C1s subcomponent(C1S) ELISA kit

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中文名稱：

人補體成分C1s(C1s)酶聯免疫試劑盒
貨號：

CSB-EL003657HU
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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人補體成分C1s(C1s)酶聯免疫試劑盒（CSB-EL003657HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C1S含量。C1S是一種重要靶點。它參與補體系統激活，在免疫防御等方面有重要作用。研究機制方面聚焦于其結構與功能關系，探索調控其活性途徑以開發治療方法，有望用于免疫相關疾病如自身免疫病的干預。試劑盒檢測范圍為25 ng/mL-1600 ng/mL，適用于基礎科研領域研究補體系統功能、探索自身免疫性疾病（如系統性紅斑狼瘡）、感染性疾病或神經退行性病變中的補體活化機制，同時可為藥物開發中補體通路調控效果的體外評估提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Basic proline rich peptide IB 1 ELISA Kit; C1 esterase ELISA Kit; C1S ELISA Kit; C1S_HUMAN ELISA Kit; Complement C1s subcomponent ELISA Kit; Complement C1s subcomponent heavy chain ELISA Kit; Complement C1s subcomponent light chain ELISA Kit; Complement component 1 s subcomponent ELISA Kit; Complement component 1 subcomponent s ELISA Kit; FLJ44757 ELISA Kit
縮寫：

C1S
Uniprot No.：

P09871
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

25 ng/mL-1600 ng/mL
靈敏度：

6.25 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C1S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	100
	Range %	91-104
1:200	Average %	107
	Range %	98-110
1:400	Average %	88
	Range %	82-93
1:800	Average %	96
	Range %	87-99

回收率：

The recovery of human C1S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	110	98-114
EDTA plasma (n=4)	98	90-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1600	2.740	2.685	2.713	2.598
800	2.346	2.276	2.311	2.196
400	1.488	1.454	1.471	1.356
200	0.756	0.768	0.762	0.647
100	0.396	0.411	0.404	0.289
50	0.246	0.239	0.243	0.128
25	0.162	0.170	0.166	0.051
0	0.113	0.117	0.115

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation Charlotte H Hulme.et al,Arthritis Research and Therapy,2018

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功能：

C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.
基因功能參考文獻：
1. TNT003, an inhibitor of the serine protease C1s, prevents complement activation induced by cold agglutinins. PMID: 24695853
2. Data indicate that complement C1s mRNA level was low in ICR-derived glomerulonephritis (ICGN) mice liver as compared with age-matched ICR mice. PMID: 23989031
3. A molecular switch governs the interaction between the human complement protease C1s and its substrate, complement C4. PMID: 23592783
4. Four positively charged amino acids on the serine protease domain appear to form a catalytic exosite that is required for efficient cleavage of C4 in the classical pathway of complement. PMID: 22855709
5. Detailed mapping of post-translational modifications and insights into the C1r/C1s binding sites. PMID: 20008834
6. Interaction with the prime side residues at the cleavage point in C1s enhances the affinity of the enzyme for complement 2 and complement 4 substrates; these prime subsite residues mediate positive cooperativity in the cleavage of the substrate. PMID: 14674770
7. full specificity of the enzyme using a randomized phage display library PMID: 16169853
8. There are splice variants of C1s mRNA transcripts in normal human cells PMID: 18062908
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相關疾病：

Complement component C1s deficiency (C1SD); Ehlers-Danlos syndrome, periodontal type, 2 (EDSPD2)
蛋白家族：

Peptidase S1 family
數據庫鏈接：

HGNC: 1247

OMIM: 120580

KEGG: hsa:716

STRING: 9606.ENSP00000328173

UniGene: Hs.458355

Human Complement C1s subcomponent(C1S) ELISA kit

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