Human Coll2-1 ELISA kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人Coll2-1酶聯免疫試劑盒（CSB-EQ027311HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液樣本中的Coll2-1含量。試劑盒檢測范圍為47 pg/mL-3000 pg/mL，Coll2-1是關節軟骨中II型膠原蛋白特異性降解的生物標志物，其水平變化與關節退行性疾病及軟骨代謝異常密切相關，在骨關節炎機制研究、藥物開發療效評估及運動醫學相關科研領域具有重要應用價值。試劑盒包含預包被抗體、標準品及檢測所需試劑，操作流程標準化，適用于體外檢測科研樣本中Coll2-1的動態變化，可為軟骨代謝研究、疾病模型建立及生物標志物篩選提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

Coll2-1
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates
檢測范圍：

47 pg/mL-3000 pg/mL
靈敏度：

11.75 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Coll2-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	85
	Range %	80-90
1:200	Average %	98
	Range %	90-105
1:400	Average %	95
	Range %	90-100
1:800	Average %	92
	Range %	87-97

回收率：

The recovery of human Coll2-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-95
EDTA plasma (n=4)	98	92-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.