Human Chitinase-3-like protein 2(CHI3L2) ELISA kit

Human Chitinase-3-like protein 2(CHI3L2) ELISA kit

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中文名稱：

人幾丁質酶3樣蛋白2(CHI3L2)酶聯免疫試劑盒
貨號：

CSB-EL005347HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人幾丁質酶3樣蛋白2(CHI3L2)酶聯免疫試劑盒（CSB-EL005347HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CHI3L2含量。CHI3L2是一種糖基水解酶家族成員，缺乏酶活性，通過結合多糖和細胞表面受體調控炎癥、組織重塑和腫瘤微環境。在關節炎、纖維化疾病和惡性腫瘤中高表達，是疾病進展的生物標志物和潛在治療靶點。試劑盒檢測范圍為0.78 ng/ml-50 ng/ml，該產品可為炎癥性疾病機制研究、纖維化模型評估或腫瘤微環境分析提供關鍵生物標志物檢測支持，特別適用于基礎醫學、免疫學及分子病理學領域的體外樣本檢測，幫助研究者探索CHI3L2在疾病發生發展中的調控作用。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CH3L2_HUMAN ELISA Kit; CHI3L2 ELISA Kit; Chitinase 3 like 2 ELISA Kit; Chitinase-3-like protein 2 ELISA Kit; Chondrocyte protein 39 ELISA Kit; OTTHUMP00000014028 ELISA Kit; YKL 39 ELISA Kit; YKL-39 ELISA Kit; YKL39 ELISA Kit
縮寫：

CHI3L2
Uniprot No.：

Q15782
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 ng/ml-50 ng/ml
靈敏度：

0.19 ng/ml
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Stem Cells
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CHI3L2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-91
1:2	Average %	101
	Range %	95-105
1:4	Average %	102
	Range %	98-109
1:8	Average %	96
	Range %	91-98

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.701

2.688

2.695

2.582

2.344

2.192

2.268

2.155

12.5

1.704

1.729

1.717

1.604

6.25

1.151

1.137

1.144

1.031

3.125

0.678

0.699

0.689

0.576

1.56

0.388

0.366

0.377

0.264

0.78

0.238

0.241

0.240

0.127

0.115

0.111

0.113

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Chitinase 3-like proteins as diagnostic and prognostic biomarkers of multiple sclerosis Hinsinger G et al,Mult Scler,2015

功能：

Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity.
基因功能參考文獻：
1. This study shown that CHI3L2 is regulated differently in healthy and late onset Alzheimer"s disease brain depending on the sex. PMID: 27653898
2. Structural analysis demonstrates that YKL-39 interacts with chitooligosaccharides through hydrogen bonds and hydrophobic interactions. Thermodynamic analysis indicates that binding of chitooligosaccharide to YKL-39 is mainly driven by enthalpy. PMID: 25477513
3. These data suggest that hYKL-39 is a novel growth and differentiation factor involved in cartilage homeostasis. PMID: 23291184
4. YKL-39 possesses a chitinase-like fold, but lacks key active-site residues required for catalysis. PMID: 22742450
5. ERK1/2 phosphorylation by CHI3L2 inhibits cell mitogenesis and proliferation. PMID: 22211103
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亞細胞定位：

Secreted.
蛋白家族：

Glycosyl hydrolase 18 family
組織特異性：

Highest expression in chondrocytes, followed by synoviocytes, lung and heart. Not detected in spleen, pancreas, and liver. May also be expressed in developing brain and placenta.
數據庫鏈接：

HGNC: 1933

OMIM: 601526

KEGG: hsa:1117

STRING: 9606.ENSP00000358763

UniGene: Hs.514840

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.