Human Centromere protein K(CENPK) ELISA kit

Human Centromere protein K(CENPK) ELISA kit

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中文名稱：

人著絲粒蛋白K(CENPK)酶聯免疫試劑盒
貨號：

CSB-EL005214HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人著絲粒蛋白K(CENPK)酶聯免疫試劑盒（CSB-EL005214HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CENPK含量。CENPK（著絲粒蛋白K）是一種與著絲粒復合體相關的亞基，其功能是將CENPA蛋白靶向到著絲粒，對于著絲粒功能和有絲分裂進程至關重要。研究顯示，CENPK基因在肝細胞癌中異常高表達，其啟動子區域的DNA低甲基化水平與其高表達密切相關，提示表觀遺傳學機制在肝細胞癌發生發展中起重要作用。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，可在科研場景中用于探索CENPK在細胞增殖、分化及癌癥發生發展中的分子機制，尤其適用于染色體動力學研究、腫瘤模型構建以及藥物干預對染色體穩定性影響的體外實驗，適用于分子生物學、腫瘤學等領域的蛋白功能研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CENPK ELISA Kit; ICEN37 ELISA Kit; FKSG14 ELISA Kit; Centromere protein K ELISA Kit; CENP-K ELISA Kit; Interphase centromere complex protein 37 ELISA Kit; Protein AF-5alpha ELISA Kit; p33 ELISA Kit
縮寫：

CENPK
Uniprot No.：

Q9BS16
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

23.5 pg/mL-1500 pg/mL
靈敏度：

5.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CENPK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	100
	Range %	90-105
1:4	Average %	92
	Range %	85-96
1:8	Average %	92
	Range %	84-96

回收率：

The recovery of human CENPK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-95
EDTA plasma (n=4)	99	90-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	1.889	1.966	1.928	1.798
750	1.426	1.476	1.451	1.321
375	0.921	0.900	0.911	0.781
187.5	0.566	0.543	0.555	0.425
94	0.369	0.345	0.357	0.227
47	0.259	0.263	0.261	0.131
23.5	0.199	0.192	0.196	0.066
0	0.131	0.129	0.130

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore.
相關疾病：

Chromosomal aberrations involving CENPK are a cause of acute leukemias. Translocation t(5;11)(q12;q23) with KMT2A/MLL1.
亞細胞定位：

Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex.
蛋白家族：

CENP-K/MCM22 family
組織特異性：

Detected in several fetal organs with highest levels in fetal liver. In adults, it is weakly expressed in lung and placenta.
數據庫鏈接：

HGNC: 29479

OMIM: 611502

KEGG: hsa:64105

STRING: 9606.ENSP00000242872

UniGene: Hs.529778

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.