Human Cathelicidin antimicrobial peptide(CAMP) ELISA kit

產品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人抗菌肽(CAMP)酶聯免疫試劑盒（CSB-EL004476HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CAMP含量。CAMP（環腺苷酸）是細胞內重要的第二信使，參與多種生理過程。研究顯示，失控的cAMP可能導致肝癌，其機制涉及cAMP在無膜細胞器中的動態隔離，當這些結構被破壞時，cAMP會淹沒細胞，導致失控生長。試劑盒檢測范圍為0.45 ng/mL-30 ng/mL，適用于體外科研實驗中探索CAMP在感染性病原體應答、慢性炎癥性疾病（如銀屑病、炎癥性腸病）或皮膚創傷愈合等生理病理過程中的表達變化，為免疫機制研究及潛在生物標志物分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CAP18, CRAMP, FALL-39, FALL39, HSD26, LL37,
縮寫：

CAMP
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.45 ng/mL-30 ng/mL
靈敏度：

0.11 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
Range %	80-92
1:200	Average %	96
Range %	91-105
1:400	Average %	99
Range %	92-110
1:800	Average %	95
Range %	86-98

回收率：

The recovery of human CAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	89-98
EDTA plasma (n=4)	95	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.