Human Cartilage acidic protein 1(CRTAC1) ELISA kit

Human Cartilage acidic protein 1(CRTAC1) ELISA kit

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中文名稱：

人軟骨酸性蛋白1(CRTAC1)酶聯(lián)免疫試劑盒
貨號：

CSB-EL005997HU
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人軟骨酸性蛋白1(CRTAC1)酶聯(lián)免疫試劑盒（CSB-EL005997HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CRTAC1含量。試劑盒檢測范圍為4.68 ng/mL-300 ng/mL，該產(chǎn)品適用于軟骨相關(guān)疾病機制研究、生物標(biāo)志物篩選及藥物干預(yù)效果評估等科研領(lǐng)域，尤其適合在血清、血漿等復(fù)雜基質(zhì)中分析CRTAC1的動態(tài)變化，為軟骨代謝研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

68 kDa chondrocyte expressed protein ELISA Kit; 68 kDa chondrocyte-expressed protein ELISA Kit; Acidic secreted protein in cartilage ELISA Kit; ASPIC ELISA Kit; ASPIC1 ELISA Kit; Cartilage acidic protein 1 ELISA Kit; Cartilage acidic protein 1 precursor ELISA Kit; CEP 68 ELISA Kit; CEP-68 ELISA Kit; CEP68 ELISA Kit; Chondrocyte expressed protein 68 kDa CEP 68 ELISA Kit; CRAC1_HUMAN ELISA Kit; CRTAC1 ELISA Kit; Protein CRTAC1-B ELISA Kit; W307 ELISA Kit
縮寫：

CRTAC1
Uniprot No.：

Q9NQ79
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

4.68 ng/mL-300 ng/mL
靈敏度：

1.17 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CRTAC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	91
	Range %	87-96
1:10	Average %	97
	Range %	93-101
1:20	Average %	102
	Range %	99-103
1:40	Average %	88
	Range %	86-91

回收率：

The recovery of human CRTAC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-93
EDTA plasma (n=4)	102	98-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
300	2.522	2.735	2.629	2.444
150	2.043	2.102	2.073	1.888
75	1.317	1.315	1.316	1.131
37.5	0.875	0.857	0.866	0.681
18.75	0.470	0.445	0.458	0.273
9.37	0.366	0.380	0.373	0.188
4.68	0.254	0.252	0.253	0.068
0	0.186	0.184	0.185

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Identification of Cardiometabolic Protein Biomarkers for Acute Myocardial Infarction Using Olink Proteomics X Tan, X Wang, S Xu, Y Zeng, G Zhang,Journal of Inflammation Research,2025

基因功能參考文獻(xiàn)：
1. Confirm the increase of CRTAC1 in cartilage biopsies from OA patients undergoing joint replacement by real-time PCR and immunohistochemistry. Furthermore, we report that proinflammatory cytokines interleukin-1beta and tumor necrosis factor alpha upregulate CRTAC1 expression in primary articular chondrocytes and synovial fibroblasts. PMID: 27415616
2. It was shown for the first time the propensity of CRTAC1 to form amyloid-like structures, and it was hypothesized that the aggregating property of CRTAC1 may be related to its disease-association. PMID: 27862299
3. These results provided new insights into the mechanism of cataract development, and demonstrated that CRTAC1 could be a potentially novel target for cataract treatment. PMID: 27855397
4. CRTAC1 acquired an alternate last exon from the tail-to-tail oriented neighbouring gene in humans resulting in the glycosylated isoform CRTAC1-A which represents a new extracellular matrix molecule of articular cartilage. PMID: 17074475
5. in bone fracture patients, 12 proteins were related to bone/cartilage metabolism, including: TGF-beta induced protein IG-H(3), cartilage acidic protein 1, procollagen C proteinase enhancer protein and TGF-beta receptor III. PMID: 17602227
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亞細(xì)胞定位：

Secreted, extracellular space, extracellular matrix.
組織特異性：

Expressed in the interterritorial matrix of articular deep zone cartilage (at protein level). Isoform 1 and isoform 2 are expressed in brain. Isoform 1 is detected in lung and chondrocytes. Detected in cartilage, bone, cultured chondrocytes and lung, and
數(shù)據(jù)庫鏈接：

HGNC: 14882

OMIM: 606276

KEGG: hsa:55118

STRING: 9606.ENSP00000359629

UniGene: Hs.500736

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.