Human Carboxypeptidase E(CPE) ELISA kit
-
中文名稱：人羧肽酶E(CPE)酶聯免疫試劑盒
-
貨號：CSB-EL005886HU
-
規格：96T/48T
-
價格：￥3600/￥2500
-
其他：
產品詳情
-
產品描述：人羧肽酶E(CPE)酶聯免疫試劑盒（CSB-EL005886HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CPE含量。CPE即羧肽酶E，是一種與神經內分泌相關的酶。其背景在于在腦和內分泌組織中廣泛存在。研究機制上，它參與神經肽和激素前體加工，對調節神經遞質釋放、內分泌信號傳導等有重要作用，在神經生理和內分泌相關疾病研究方面受關注。試劑盒檢測范圍為39.06 pg/mL-2500 pg/mL，適用于科研場景下探究CPE在代謝性疾病模型、神經內分泌調控機制中的表達變化，亦可用于評估藥物干預或基因修飾對CPE活性的影響。為研究CPE在病理生理過程中的分子機制及其作為潛在生物標志物的探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
-
別名：Carboxypeptidase E ELISA Kit; Carboxypeptidase H ELISA Kit; Carboxypeptidase H precursor ELISA Kit; CarboxypeptidaseE ELISA Kit; CarboxypeptidaseH ELISA Kit; CBPE_HUMAN ELISA Kit; Cobalt stimulated chromaffin granule carboxypeptidase ELISA Kit; CPE ELISA Kit; Cph 1 ELISA Kit; CPH ELISA Kit; Enkephalin convertase ELISA Kit; Entephalin convertase ELISA Kit; Insulin granule associated carboxypeptidase ELISA Kit; Prohormone processing carboxypeptidase ELISA Kit; Prohormone-processing carboxypeptidase ELISA Kit
-
縮寫：CPE
-
Uniprot No.：
-
種屬：Homo sapiens (Human)
-
樣本類型：serum, plasma, tissue homogenates
-
檢測范圍：39.06 pg/mL-2500 pg/mL
-
靈敏度：9.76 pg/mL
-
反應時間：1-5h
-
樣本體積：50-100ul
-
檢測波長：450 nm
-
研究領域：Metabolism
-
測定原理：quantitative
-
測定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human CPE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 105 Range % 94-109 1:2 Average % 107 Range % 98-111 1:4 Average % 92 Range % 84-95 1:8 Average % 98 Range % 90-102 -
回收率：
The recovery of human CPE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 84-94 EDTA plasma (n=4) 101 95-104 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 2500 2.410 2.333 2.372 2.246 1250 1.712 1.679 1.696 1.570 625 1.029 0.968 0.999 0.873 312.5 0.634 0.655 0.645 0.519 156.25 0.410 0.401 0.406 0.280 78.12 0.280 0.284 0.282 0.156 39.06 0.218 0.225 0.222 0.096 0 0.125 0.127 0.126 -
數據處理：
-
貨期：3-5 working days
產品評價
相關產品
靶點詳情
-
功能：Sorting receptor that directs prohormones to the regulated secretory pathway. Acts also as a prohormone processing enzyme in neuro/endocrine cells, removing dibasic residues from the C-terminal end of peptide hormone precursors after initial endoprotease cleavage.
-
基因功能參考文獻：
- Our results implicate a novel role for Carboxypeptidase E that mainly affects the expression of motility-associated genes via several signal pathways PMID: 28656234
- This study has uncovered a human CPE/NF-alpha1 gene mutation that could lead to comorbidity of dementia and depression, emphasizing the importance of this gene in cognitive function. PMID: 27922637
- CPE through its N'-terminal sequence, forms aggregates with Wnt3a and possible endoplasmic reticulum (ER) stress leading to its loss of function. PMID: 27375026
- we have identified a novel SNP in the CPE gene which results in the loss of its neuroprotective function in cells and may confer neurological disorders in humans PMID: 28114332
- High-level CPE [ carboxypeptidase E] expression was associated with a poor prognosis in early-stage cervical cancer. CPE may serve as a biomarker for predicting PLNM [ pelvic lymph node metastasis ] and survival in these patients. PMID: 26695643
- Low carboxypeptidase E expression is associated with recurrence in early-stage hepatocellular carcinoma. PMID: 26803519
- Down-expression of liver carboxypeptidase E may reduce the secretion of serum cholecystokinin and contribute to the formation of cholesterol gallstone. PMID: 26228366
- Downregulation of CPE regulates cell proliferation and chemosensitivity in pancreatic cancer. PMID: 25374060
- Disruption of insulin receptor (IR) expression in beta cells has a direct impact on the expression of the convertase enzyme carboxypeptidase E (CPE) by inhibition of the eukaryotic translation initiation factor Eif4g1. PMID: 24843127
- Upregulation of CPE promotes cell proliferation and tumorigenicity in colorectal cancer. PMID: 24006921
- Data suggest that splice variant of carboxypeptidase E (CPE-DeltaN) that CPE-DeltaN expression might be a potential prognostic marker for colorectal cancer patients. PMID: 23852859
- CPE is essential in the process and targeting of neuropeptides and neurotrophins, its participation in the pathological progression of Alzheimer's disease may be suggested PMID: 22998035
- CPE forms a complex, probably through sequences located at its N-terminal domain, with Wnt3a and the extracellular cysteine rich domain of Fz1. PMID: 22824791
- Neither high glucose nor insulin (with low glucose) regulates beta-cell CPE (but either up-regulates CPD). PMID: 21628999
- an N-terminal truncated carboxypeptidase E splice isoform induces tumor growth and is a biomarker for predicting future metastasis in human cancers PMID: 21285511
- CPE may play a role in promoting tumor growth and invasion. PMID: 21061162
- Carboxypeptidase E is differently expressed in subcutaneous and visceral fat of obese subjects PMID: 12530526
- protein binding with Con A in seminal plasma PMID: 14690244
- cDNA microarray analysis led to the identification of 2 novel biomarkers that should facilitate molecular diagnosis and further study of pulmonary neuroendocrine tumors. PMID: 15492986
- A possible role for mutations in CPE in the development of coronary heart disease. PMID: 17957445
- the severity of the coronary atherosclerosis estimated by Gensini score was significantly influenced by the presence of the A2925G mutant and G2855A mutant of the CPE gene PMID: 18080843
- polymorphism in the CPE exon5 gene may contribute to the angiographical characteristics of coronary atherosclerosis in the Chinese population PMID: 18501121
- Carboxypeptidase E degradation contributes to palmitate-induced beta-cell ER stress and apoptosis; CPE is a major link between hyperlipidemia and beta-cell death pathways in diabetes. PMID: 18550819
- CPH-Abs may allow discrimination of a more latent subset of adult-onset autoimmune diabetes (LADA). PMID: 19120309
- Carboxypeptidase E may be a key molecule to regulate Caspr2 (carboxypeptidase E) trafficking to the cell membrane. PMID: 19166515
- missense polymorphism encoding altered activity PMID: 11462236
顯示更多
收起更多
-
亞細胞定位：[Isoform 1]: Cytoplasmic vesicle, secretory vesicle. Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein. Secreted.
-
蛋白家族：Peptidase M14 family
-
數據庫鏈接：
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-
