Human Carboxymethyl lysine,CML ELISA Kit

產品詳情

文獻引用 (13) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人羧甲基賴氨酸(CML)酶聯免疫試劑盒（CSB-E12798h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CML含量。CML即慢性髓系白血病，是一種起源于多能造血干細胞的血液系統惡性克隆性疾病，與費城染色體及BCR-ABL融合基因相關。研究機制聚焦于該基因異常導致的酪氨酸激酶持續活化，目前多通過靶向抑制該激酶來開展治療。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于基礎科研中AGEs代謝機制探索、抗衰老藥物功效評估以及糖尿病相關動物模型或細胞實驗的分子標記物檢測。其寬泛的樣本兼容性支持跨組織類型的比較研究，為營養干預、代謝性疾病機理及氧化損傷相關領域提供可靠工具。助力研究者深入解析CML在慢性疾病發生發展中的生物學作用。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

CML
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CML in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-95
1:2	Average %	94
	Range %	90-100
1:4	Average %	95
	Range %	91-105
1:8	Average %	94
	Range %	85-100

回收率：

The recovery of human CML spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-98
EDTA plasma (n=4)	94	91-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














pg/ml	OD1	OD2	Average	Corrected
4000	2.372	2.252	2.312	2.162
2000	1.775	1.660	1.718	1.568
1000	1.160	1.142	1.151	1.001
500	0.712	0.699	0.706	0.556
250	0.493	0.456	0.475	0.325
125	0.342	0.329	0.336	0.186
62.5	0.264	0.249	0.257	0.107
0	0.153	0.147	0.150